Subcellular clustering of a putative c-di-GMP-dependent exopolysaccharide machinery affecting macro colony architecture in Bacillus subtilis
Subcellular clustering of a putative c-di-GMP-dependent exopolysaccharide machinery affecting macro colony architecture in Bacillus subtilis
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DOI：
10.1111/1758-2229.12496
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发表时间：
2017-06-01
影响因子：
3.3
通讯作者：
Graumann, Peter L.
中科院分区：
文献类型：
--
作者：
Bedrunka, Patricia;Graumann, Peter L.
细菌生物膜的结构主要是通过胞外聚合物（EPS）的分泌建立的。他们表明，枯草芽孢杆菌含有操纵子（ydaJ-N），其诱导导致生物膜刚果红染色增加并强烈改变生物膜结构，表明它介导未知胞外多糖的产生。支持这一观点的是，YdaJKLMN 的过量产生会导致液体培养中指数生长期间的细胞聚集，并且还会导致野生型细胞以及缺乏枯草芽孢杆菌主要胞外多糖的突变背景中的集落形态改变。操纵子的第一个基因产物 YdaJ 似乎可以改变过度生产的影响，但对于细胞聚集或改变集落形态来说并不是必需的，而 c-di-GMP 受体 YdaK 的存在是必需的，这表明第二信使 c-di-GMP 的参与。 YdaM、YdaN 和 YdaK 主要在细胞极共定位于簇，并静态定位在该亚细胞位点，类似于其他细菌中的其他胞外多糖机械组件。他们的分析表明，枯草芽孢杆菌含有影响细胞聚集和生物膜形成的 EPS 机制的静态亚细胞组装。
The structure of bacterial biofilms is predominantly established through the secretion of extracellular polymeric substances (EPS). They show that Bacillus subtilis contains an operon (ydaJ-N) whose induction leads to increased Congo Red staining of biofilms and strongly altered biofilm architecture, suggesting that it mediates the production of an unknown exopolysaccharide. Supporting this idea, overproduction of YdaJKLMN leads to cell clumping during exponential growth in liquid culture, and also causes colony morphology alterations in wild type cells, as well as in a mutant background lacking the major exopolysaccharide of B. subtilis. The first gene product of the operon, YdaJ, appears to modify the overproduction effects, but is not essential for cell clumping or altered colony morphology, while the presence of the c-di-GMP receptor YdaK is required, suggesting an involvement of second messenger c-di-GMP. YdaM, YdaN and YdaK colocalize to clusters predominantly at the cell poles and are statically positioned at this subcellular site, similar to other exopolysaccharide machinery components in other bacteria. Their analysis reveals that B. subtilis contains a static subcellular assembly of an EPS machinery that affects cell aggregation and biofilm formation.