A 4-year study of plasma ochratoxin A in a selected population in Tokyo by immunoassay and immunoaffinity column-linked HPLC.
A 4-year study of plasma ochratoxin A in a selected population in Tokyo by immunoassay and immunoaffinity column-linked HPLC.
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通过免疫测定和免疫亲和柱连接 HPLC 对东京选定人群中的血浆赭曲霉毒素 A 进行了为期 4 年的研究。
DOI：
10.1016/s0278-6915(98)00004-0
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发表时间：
1998
影响因子：
4.3
通讯作者：
O. Kawamura
中科院分区：
文献类型：
--
作者：
Yoshio Ueno;S. Maki;J. Lin;M. Furuya;Y. Sugiura;O. Kawamura
采用以单抗为基础的竞争酶联免疫吸附试验(CELISA)和新型免疫亲和柱(IAC)连接的高效液相色谱-荧光法，对东京地区184名健康志愿者(男130名，女54名)的血浆赭曲霉毒素A(OTA)浓度进行了检测。结果表明，除1994年38%的病例外，85%的病例OTA呈阳性，平均阳性值为68pg/ml，这表明东京人群接触OTA的频率很高，尽管其血浆水平远低于欧洲和加拿大的报道。发酵的饮食食品、咖啡和葡萄酒的部分贡献被怀疑是在人体血浆中观察到的OTA的来源。
Employing a competitive ELISA (cELISA) based on monoclonal antibody and a novel immunoaffinity-column (IAC)-linked HPLC-fluorometry, ochratoxin A (OTA) levels in the plasma of 184 healthy volunteers (130 males, 54 females) were surveyed in Tokyo during the 4 years from 1992 to 1996. It was found that 85% of the cases were positive for OTA except 38% in 1994, and an average value in the positives was estimated as 68pg/ml. This suggests that the population in Tokyo is exposed to OTA at high frequency, although the level in plasma is far less than that reported in Europe and Canada. A partial contribution of fermented dietary foodstuffs, coffee and wine is suspected as the source of the OTA observed in the human plasma.