Production and characterization of the neutralization antigen VP2 of bluetongue virus serotype 10 using a baculovirus expression vector.
Production and characterization of the neutralization antigen VP2 of bluetongue virus serotype 10 using a baculovirus expression vector.
复制标题
DOI：
10.1016/0042-6822(87)90289-3
复制
发表时间：
1987-04
期刊：
影响因子：
3.7
通讯作者：
S. Inumaru;P. Roy
中科院分区：
文献类型：
--
作者：
S. Inumaru;P. Roy
代表蓝舌病毒(BTV)10型RNA片段2的DNA已被插入杆状病毒转移载体中，取代了银纹夜蛾核型多角体病毒(AcNPV)多角体基因的5‘编码区。在所构建的重组转移载体DNA存在下，将野生型AcNPV DNA与果翅夜蛾细胞共转染后，获得了重组杆状病毒。什么时候。用其中一种重组病毒感染果树细胞，合成了一种大小和抗原性与BTV VP2蛋白相似的蛋白。表达VP2蛋白的小鼠和兔的抗体可中和BTV-10病毒的感染性，对BTV-11和17型病毒的感染性较小，但不能中和BTV-13病毒的感染性。
DNA representing RNA segment 2 of bluetongue virus (BTV) serotype 10, corresponding to the gene that codes for the BTV neutralization antigen VP2, has been inserted into a baculovirus transfer vector in lieu of the 5′ coding region of the polyhedrin gene ofAutographa californicanuclear polyhedrosis virus (AcNPV). After cotransfection ofSpodoptera frugiperdacells with wild-type AcNPV DNA in the presence of the derived recombinant transfer vector DNA, polyhedrin-negative recombinant baculoviruses were recovered. WhenS. frugiperdacells were infected with one of these recombinant viruses, a protein that was similar in size and antigenic properties to the BTV VP2 protein was synthesized. Antibodies raised in mice or rabbits to the baculovirus expressed VP2 protein neutralized the infectivity of BTV-10 virus and to lesser extents BTV serotype 11 and 17 viruses but not BTV-13 virus.