Involvement of Porphyromonas gingivalis fimA genotype in treatment outcome following non-surgical periodontal therapy
Involvement of Porphyromonas gingivalis fimA genotype in treatment outcome following non-surgical periodontal therapy
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DOI：
10.1902/jop.2005.76.10.1661
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发表时间：
2005-10-01
影响因子：
4.3
通讯作者：
Maeda, K
中科院分区：
文献类型：
--
作者：
Fujise, O;Miura, M;Maeda, K
背景：牙龈卟啉单胞菌被认为是牙周病的重要致病菌。根据编码菌毛蛋白的fimA基因的多样性将其分为6种基因型。本研究评估参与fimA基因型在治疗结果以下非手术牙周therapy.Methods：慢性牙周炎患者参加了这项研究，所有接受临床和微生物学检查在基线。采用聚合酶链反应方法检测龈下菌群并鉴定牙龈卟啉单胞菌fimA基因型。总共接受了160个牙龈卟啉单胞菌阳性部位，这些部位具有探诊出血（BOP）和A mm的探诊深度。结果：纵向调查显示，fimA Ⅰ型阳性者治疗后持续性BOP的发生率明显高于Ⅰ型阴性者（51.6%比27.9%），而Ⅰ b型和Ⅱ 1型患者治疗后持续性BOP的发生率明显高于Ⅰ型阴性者（51.6%比27.9%）。I型阳性位点在治疗后也显示出比I型阴性位点更持久的Tannerella lasthensis和P. gingivalis。在治疗后的调查中，I型阳性部位的BOP和T的频率较高。结论：治疗后Ⅰ型阳性部位BOP改善不明显，Ⅰ型和T型fimA的联合持续存在可能是由于Ⅰ型和T型fimA的共同作用。这种不良的治疗结果似乎与Escherichensis有关。本研究证明了牙龈卟啉单胞菌fimA I型作为治疗后持续性BOP的预测因子的潜力。
Background: Porphyromonas gingivalis is considered a critical pathogen in periodontal diseases. It is classified into six genotypes based on diversity of the fimA gene encoding fimbrillin. The present study evaluated the involvement of the fimA genotype in treatment outcome following non-surgical periodontal therapy.Methods: Chronic periodontitis patients were enrolled in this study; all received clinical and microbiological examinations at baseline. The detection of subgingival species and identification of P. gingivalis fimA genotypes were performed using polymerase chain reaction based methods. In total, 160 P. gingivalis positive sites with bleeding on probing (BOP) and a probing depth of A mm were accepted. They were followed up after scaling and root planing.Results: Longitudinal investigation indicated that fimA type I positive sites at baseline were followed by a significantly higher frequency of persistent BOP after treatment than type I negative sites (51.6% versus 27.9%), while types Ib and 11 were not. Type I positive sites also showed more persistence of Tannerella forsythensis and P. gingivalis after treatment than type I negative sites. In post-treatment investigation, type I positive sites showed higher frequencies of BOP and T. forsythensis detection than type I negative sites (77.8% versus 43.5% and 100% versus 76.1%, respectively).Conclusions: BOP in initially type I positive sites showed little improvement with treatment, and the combined persistence of fimA type I and T. forsythensis seemed to be involved in this poor treatment outcome. The present study demonstrated the potential of P. gingivalis fimA type I as a predictor of persistent BOP after treatment.