ALTERNATIVE SPLICING GENERATES METABOTROPIC GLUTAMATE RECEPTORS INDUCING DIFFERENT PATTERNS OF CALCIUM RELEASE IN XENOPUS OOCYTES
ALTERNATIVE SPLICING GENERATES METABOTROPIC GLUTAMATE RECEPTORS INDUCING DIFFERENT PATTERNS OF CALCIUM RELEASE IN XENOPUS OOCYTES
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DOI：
10.1073/pnas.89.21.10331
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发表时间：
1992-11-01
影响因子：
11.1
通讯作者：
HEINEMANN, SF
中科院分区：
文献类型：
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作者：
PIN, JP;WAEBER, C;HEINEMANN, SF
代谢型谷氨酸受体（mGluR）1a的剪接变体被分离，命名为mGluR 1c。与mGluR 1a相比，预测的mGluR 1c蛋白在其C-末端短302个氨基酸。尽管有这种差异，mGluR 1c激活磷脂酶C在爪蟾卵母细胞的药理学概况相同的mGluR 1a。然而，在相反的大快速瞬态反应诱导mGluR 1a，mGluR 1c受体引起一个小的更缓慢地产生持久的振荡电流，这表明这两个受体不产生相同的模式的Ca 2+释放爪蟾卵母细胞。原位杂交数据显示，mGluR 1c mRNA的表达水平低于mGluR 1的其他剪接变体。在小脑、嗅球和纹状体中观察到这些转录本的区域分布的一些差异。
A splice variant of the metabotropic glutamate receptor (mGluR) 1a, named mGluR1c, was isolated. Compared to mGluR1a, the predicted mGluR1c protein is 302 amino acids shorter at its C-terminal end. Despite this difference, mGluR1c activates phospholipase C in Xenopus oocytes with a pharmacological profile identical to that of mGluR1a. However, in contrast to the large fast transient responses induced by mGluR1a, mGluR1c receptors elicit a small more slowly generated long-lasting oscillatory current, suggesting that these two receptors do not generate the same pattern of Ca2+ release in Xenopus oocytes. In situ hybridization data show that mGluR1c mRNA is expressed at a lower level than the other splice variants of mGluR1. Some differences in the regional distribution of these transcripts were observed in the cerebellum, the olfactory bulb, and the striatum.