Microbial diversity and fermentation profile of red clover silage inoculated with reconstituted indigenous and exogenous epiphytic microbiota
Microbial diversity and fermentation profile of red clover silage inoculated with reconstituted indigenous and exogenous epiphytic microbiota
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接种重组本土和外源附生微生物群的红三叶草青贮饲料的微生物多样性和发酵概况
DOI：
10.1016/j.biortech.2020.123606
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发表时间：
2020-10-01
影响因子：
11.4
通讯作者：
Shao, Tao
中科院分区：
文献类型：
--
作者：
Ali, Niaz;Wang, Siran;Shao, Tao
研究了内生和外源附生菌群移植重组对红三叶草青贮发酵品质和微生物群落的影响。用蒸馏水（RC 0）、提取的红三叶草（RC）、玉米（MZ）和高粱（SG）的附生微生物群接种无菌红三叶草。RC接种迅速降低pH值的青贮开始。在整个青贮过程中，RC青贮的LA浓度和LA/AA比值较高，而MZ青贮在青贮后期较高。RC早期青贮以片球菌属为主，MZ后期青贮以乳酸菌为主。SG终端青贮具有较高的pH（> 4.50），并且以鞘氨醇单胞菌属、肠杆菌属和新鞘氨醇菌属为主。RC和MZ菌群有利于提高红三叶草青贮发酵品质和微生物群落。附生微生物的移植和重建可以是评估目标作物的有效和生态友好的添加剂的成功方法。
The study investigated the effects of transplantation and reconstitution of indigenous and exogenous epiphytic microbiota on the fermentation quality and microbial community of red clover silage. Sterile red clover was inoculated with distilled water (RC0), extracted epiphytic microbiota of red clover (RC), maize (MZ), and sorghum (SG). RC inoculation rapidly decreased pH at the onset of ensiling. The LA concentration and ratio of LA/AA were higher in RC silage during entire ensiling while MZ silage during late phase of ensiling. Pediococcus was dominant in RC early silage, while Lactobacillus was abundant in MZ final silage. The SG terminal silage had higher pH (> 4.50) and dominated by Sphingomonas, Enterobacter, and Novosphingobium. RC and MZ microbiota were beneficial in enhancing fermentation quality and microbial community in red clover silage. Transplantation and reconstitution of epiphytic microbiota can be a successful method to assess the effective and eco-friendly additive for the targeted crop.