Generation and characterization of a novel human iPSC line from a resilient Alzheimer's disease patient
Generation and characterization of a novel human iPSC line from a resilient Alzheimer's disease patient
复制标题
DOI：
10.1016/j.scr.2020.101979
复制
发表时间：
2020-10-01
影响因子：
1.2
通讯作者：
Mahairaki, Vasiliki
中科院分区：
文献类型：
--
作者：
Das, Debamitra;Li, Jiaxin;Mahairaki, Vasiliki
阿尔茨海默病（AD）是一种进行性神经退行性疾病，是老年人痴呆的主要原因。在这里，我们报道了一名80岁的AD患者的人类诱导多能干细胞（iPSCs）的衍生，该患者具有APOE epsilon 4/epsilon 4基因型，并且对认知能力下降有10年的恢复能力。通过短暂表达多能性基因，从该患者的血细胞中重编程的iPSC维持了epsilon 4/epsilon 4基因型，核型正常，并表现出典型的iPSC特征。分化后，iPSCs能够分化成三个胚层的细胞，证实了它们的多能性。
Alzheimer's disease (AD) is a progressive neurodegenerative disease that is the major cause of dementia in older people. Here, we report the derivation of human induced pluripotent stem cells (iPSCs) from an AD patient at age of 80 who has the APOE epsilon 4/epsilon 4 genotype and is resilient to cognitive decline for 10 years. The iPSCs reprogrammed from the blood cells of this patient by transient expression of pluripotency genes maintain the epsilon 4/epsilon 4 genotype, are karyotypically normal and display typical iPSC characteristics. Upon differentiation, the iPSCs are able to differentiate into cells of the three germ layers, confirming their pluripotency.