The effect of formononetin on the proliferation and migration of human umbilical vein endothelial cells and its mechanism
The effect of formononetin on the proliferation and migration of human umbilical vein endothelial cells and its mechanism
复制标题
芒柄花素对人脐静脉内皮细胞增殖和迁移的影响及其机制
DOI：
10.1016/j.biopha.2018.12.049
复制
发表时间：
2019-03-01
影响因子：
7.5
通讯作者：
Huang, Zhaoquan
中科院分区：
文献类型：
--
作者：
Liang, Chunmei;Zhou, Anwei;Huang, Zhaoquan
芒柄花素是中药红三叶草（Trifolium pratense L.）的主要活性成分之一。先前的研究已经证明芒柄花素是一种典型的植物雌激素，并表现出雌激素样作用，保护心血管系统，副作用最小。在本研究中，我们进一步研究芒柄花素在人脐静脉内皮细胞（HUVECs）中的作用及其潜在的分子机制。我们发现，芒柄花素促进增殖和迁移的HUVECs的MTT和伤口愈合试验评估。同时，胰岛素样生长因子1（IGF-1）和细胞间粘附分子1（ICAM-1）蛋白水平呈剂量依赖性增加。本研究结果表明芒柄花素对HUVECs具有保护作用，其分子机制可能与IGF-1 R和ICAM-1，以及上调血管内皮生长因子（VEGF）和激活细胞外信号调节激酶（ERK）有关。
Formononetin is one of the main active components of traditional Chinese medicine red clover (Trifolium pratense L). Prior studies have demonstrated that formononetin is a typical phytoestrogen and exhibits an estrogen-like effect that protects the cardiovascular system with minimal side effects. In this study, we further investigated the role of formononetin in human umbilical vein endothelial cells (HUVECs) and its potential molecular mechanisms. We founded that formononetin promotes proliferation and migration of HUVECs as assessed by the MTT and wound healing assays. Meanwhile, insulin-like growth factor 1 (IGF-1) and intercellular adhesion molecule 1 (ICAM-1) protein levels were increased in a dose-dependent manner. Our findings illustrated that formononetin exhibits a protective effect on HUVECs, and the molecular mechanisms may correlate with IGF-1R and ICAM-1, as well as upregulation of vascular endothelial growth factor (VEGF) and activation of extracellular signal-regulated kinase (ERK).