Local Auxin Biosynthesis Mediated by a YUCCA Flavin Monooxygenase Regulates Haustorium Development in the Parasitic Plant Phtheirospermum japonicum
Local Auxin Biosynthesis Mediated by a YUCCA Flavin Monooxygenase Regulates Haustorium Development in the Parasitic Plant Phtheirospermum japonicum
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DOI：
10.1105/tpc.16.00310
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发表时间：
2016-08-01
期刊：
影响因子：
11.6
通讯作者：
Shirasu, Ken
中科院分区：
文献类型：
--
作者：
Ishida, Juliane K.;Wakatake, Takanori;Shirasu, Ken
列当科寄生植物在世界范围内引起严重的农业问题。寄生植物在识别宿主释放的信号后，发育出一种多细胞的感染器官，称为吸器。为了了解与宿主信号感知和吸器发育相关的分子事件，我们使用从头组装的转录组和定制的微阵列确定了在兼性寄生虫Phtheirospermum japonicum早期吸器发育过程中表达的差异调节基因。在早期吸器发育过程中上调的基因中，我们确定了YUC3，它编码一种参与生长素生物合成的功能性YUCCA（YUC）黄素单加氧酶。YUC 3在吸器形成的早期特异性表达于寄主接触部位周围的表皮细胞。YUC3的时空表达模式与生长素反应标记DR5的时空表达模式一致，表明生长素反应最大值在吸器顶端产生。用YUC3敲除构建体转化的根形成吸器的频率低于非转基因根。此外，YUC 3在根表皮细胞的异位表达诱导了转基因大豆根中吸器样结构的形成。我们的研究结果表明，生长素生物合成基因YUC 3在表皮细胞附近的接触网站的表达起着关键作用吸器的形成在根寄生植物P.japonicum。
Parasitic plants in the Orobanchaceae cause serious agricultural problems worldwide. Parasitic plants develop a multicellular infectious organ called a haustorium after recognition of host-released signals. To understand the molecular events associated with host signal perception and haustorium development, we identified differentially regulated genes expressed during early haustorium development in the facultative parasite Phtheirospermum japonicum using a de novo assembled transcriptome and a customized microarray. Among the genes that were upregulated during early haustorium development, we identified YUC3, which encodes a functional YUCCA (YUC) flavin monooxygenase involved in auxin biosynthesis. YUC3 was specifically expressed in the epidermal cells around the host contact site at an early time point in haustorium formation. The spatio-temporal expression patterns of YUC3 coincided with those of the auxin response marker DR5, suggesting generation of auxin response maxima at the haustorium apex. Roots transformed with YUC3 knockdown constructs formed haustoria less frequently than nontransgenic roots. Moreover, ectopic expression of YUC3 at the root epidermal cells induced the formation of haustorium-like structures in transgenic P. japonicum roots. Our results suggest that expression of the auxin biosynthesis gene YUC3 at the epidermal cells near the contact site plays a pivotal role in haustorium formation in the root parasitic plant P. japonicum.