Effect of repeated forearm muscle cooling on the adaptation of skeletal muscle metabolism in humans
Effect of repeated forearm muscle cooling on the adaptation of skeletal muscle metabolism in humans
复制标题
反复前臂肌肉冷却对人体骨骼肌代谢适应的影响
DOI：
10.1007/s00484-016-1303-z
复制
发表时间：
2017
影响因子：
3.2
通讯作者：
Yutaka Tochihara
中科院分区：
文献类型：
--
作者：
Hitoshi Wakabayashi;Takayuki Nishimura;Titis Wijayanto;Shigeki Watanuki;Yutaka Tochihara
本研究旨在探讨前臂肌肉反复降温对骨骼肌代谢适应性的影响。假设肌肉温度的反复降低会增加低温骨骼肌的耗氧量。16名健康男性参与了本研究。他们的右前臂肌肉通过附着在皮肤上的冷却垫局部冷却到25 °C。这种局部冷却在不同的日子重复八次，八名参与者（实验组），而八名对照组没有接受冷暴露。为了评估骨骼肌代谢的适应性，在重复冷却期之前和之后进行局部冷却试验。在前臂肌肉温度每降低2 °C时，使用近红外光谱法测量静息和25秒等长握法（10%最大随意构造）期间趾屈肌中氧合血红蛋白含量的变化。在休息时和等长握力期间，通过上臂袖带充气阻断动脉血流15 s。通过动脉闭塞期间氧合血红蛋白变化的斜率来评估指屈肌中的氧耗。在实验组中，干预前后骨骼肌的静息耗氧量无明显差异，但从热中性基线到31 °C肌温，干预后等长握力时的肌耗氧量显著高于干预前（P< 0.05）。这一结果表明，反复的局部肌肉冷却可能促进骨骼肌中的氧化代谢。总之，在反复局部肌肉冷却后，亚最大等长握力过程中的骨骼肌代谢得到促进。
This study aimed to investigate the effect of repeated cooling of forearm muscle on adaptation in skeletal muscle metabolism. It is hypothesized that repeated decreases of muscle temperature would increase the oxygen consumption in hypothermic skeletal muscle. Sixteen healthy males participated in this study. Their right forearm muscles were locally cooled to 25 °C by cooling pads attached to the skin. This local cooling was repeated eight times on separate days for eight participants (experimental group), whereas eight controls received no cold exposure. To evaluate adaptation in skeletal muscle metabolism, a local cooling test was conducted before and after the repeated cooling period. Change in oxy-hemoglobin content in the flexor digitorum at rest and during a 25-s isometric handgrip (10% maximal voluntary construction) was measured using near-infrared spectroscopy at every 2 °C reduction in forearm muscle temperature. The arterial blood flow was occluded for 15 s by upper arm cuff inflation at rest and during the isometric handgrip. The oxygen consumption in the flexor digitorum muscle was evaluated by a slope of the oxy-hemoglobin change during the arterial occlusion. In the experimental group, resting oxygen consumption in skeletal muscle did not show any difference between pre- and post-intervention, whereas muscle oxygen consumption during the isometric handgrip was significantly higher in post-intervention than in pre-test from thermoneutral baseline to 31 °C muscle temperature (P< 0.05). This result indicated that repeated local muscle cooling might facilitate oxidative metabolism in the skeletal muscle. In summary, skeletal muscle metabolism during submaximal isometric handgrip was facilitated after repeated local muscle cooling.