The contribution of solid-state NMR spectroscopy to understanding biomineralization: Atomic and molecular structure of bone
The contribution of solid-state NMR spectroscopy to understanding biomineralization: Atomic and molecular structure of bone
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DOI：
10.1016/j.jmr.2014.12.011
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发表时间：
2015-04-01
影响因子：
2.2
通讯作者：
Duer, Melinda J.
中科院分区：
文献类型：
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作者：
Duer, Melinda J.
固态核磁共振光谱对我们理解矿化组织的结构，特别是骨骼的结构产生了重大影响。骨是矿化组织中固有的有机-无机复合结构。骨中细胞外基质的有机成分主要由胶原三螺旋分子的有序原纤维组成，其中无机成分磷酸钙颗粒由矿物质血小板堆叠组成，排列在原纤维周围。这种观点认为，我们目前的骨矿物质结构模型中的关键因素是通过核磁共振光谱学产生的，并且已经产生了关于矿物质颗粒如何与底层有机基质界面和结合的主要信息。骨或任何其他结构组织的有机基质内的胶原蛋白的结构尚未确定，但在这里，这一观点也表明，通过结合其他技术应用固态NMR光谱学，已经取得了真实的进展。特别是，NMR光谱学强调了这样一个事实，即即使在这些结构蛋白质中，也存在相当大的动力学，这表明在使用固有的静态结构模型（例如X射线衍射分析所产生的模型）以深入了解分子作用时应谨慎。很明显，核磁共振方法在这一领域仍处于起步阶段，我们可以期待未来有更多的发展，特别是在了解骨骼疾病和衰老的分子机制方面。(C)2015 Elsevier Inc. All rights reserved.
Solid-state NMR spectroscopy has had a major impact on our understanding of the structure of mineralized tissues, in particular bone. Bone exemplifies the organic-inorganic composite structure inherent in mineralized tissues. The organic component of the extracellular matrix in bone is primarily composed of ordered fibrils of collagen triple-helical molecules, in which the inorganic component, calcium phosphate particles, composed of stacks of mineral platelets, are arranged around the fibrils. This perspective argues that key factors in our current structural model of bone mineral have come about through NMR spectroscopy and have yielded the primary information on how the mineral particles interface and bind with the underlying organic matrix. The structure of collagen within the organic matrix of bone or any other structural tissue has yet to be determined, but here too, this perspective shows there has been real progress made through application of solid-state NMR spectroscopy in conjunction with other techniques. In particular, NMR spectroscopy has highlighted the fact that even within these structural proteins, there is considerable dynamics, which suggests that one should be cautious when using inherently static structural models, such as those arising from X-ray diffraction analyses, to gain insight into molecular roles. It is clear that the NMR approach is still in its infancy in this area, and that we can expect many more developments in the future, particularly in understanding the molecular mechanisms of bone diseases and ageing. (C) 2015 Elsevier Inc. All rights reserved.