The aberrant expression of CD45 isoforms and levels of sex hormones in systemic lupus erythematosus
The aberrant expression of CD45 isoforms and levels of sex hormones in systemic lupus erythematosus
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系统性红斑狼疮CD45亚型的异常表达和性激素水平
DOI：
10.1007/s10067-021-05934-x
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发表时间：
2022-01-22
影响因子：
3.4
通讯作者：
Su, Zhongjing
中科院分区：
文献类型：
--
作者：
Dong, Zhaoxia;Zhang, Bin;Su, Zhongjing
系统性红斑狼疮(SLE)是一种常见的自身免疫性疾病，在女性中具有明显的性别偏见，性激素被认为在免疫活动的调节中起着重要作用。通过选择性剪接产生的CD45亚型识别了淋巴细胞的不同功能状态，也被认为是评估SLE进展的生物标志物，而CD45在SLE患者中的表达调控尚不清楚。结果与健康对照组比较，SLE患者外周血中补体C3和IgA水平降低，尤其是血清IgA水平下降至选择性免疫球蛋白A缺乏水平，C反应蛋白升高，提示SLE患者免疫功能异常。性激素检测显示SLE患者睾酮水平降低，催乳素水平升高。SLE患者CD45RO表达加快，CD45RA和CD45RB表达降低，CD45DNA相对甲基化，为解释SLE免疫功能的可能调节机制提供了线索。结论CD45亚型、DNA甲基化和激素水平的异常可能与SLE患者免疫活性失衡有关。
IntroductionSystemic lupus erythematosus (SLE) is a common autoimmune disease with significant gender bias in women, and sex hormones are considered to play an important role in the regulation of immune activity. The CD45 isoforms generated through alternative splicing of mRNA identify different functional status of lymphocytes and also are suggested as a biomarker for assessing the progression of SLE, while the modulation of CD45 expression in SLE patients is not clear.MethodsIn this study, the peripheral blood sera of 46 SLE patients and 15 health individuals were collected for detecting the levels of sex hormones and immune associated factors. The expression of CD45 isoforms and the status of CD45 DNA methylation of the peripheral mononuclear blood cells were detected by flow cytometry and bisulfite sequencing PCR, respectively.ResultsThe levels of complement C3 and IgA decreased, especially decline of the serum IgA to the level of selective immunoglobulin A deficiency, and the C-reactive protein increased in SLE patients when compared with healthy controls, which manifested the abnormal immune activity of the SLE patients. Sex hormones detection showed a decreased testosterone and increased prolactin in SLE. An accelerated expression of CD45RO, reduced CD45RA and CD45RB, and a relative hypermethylation of CD45 DNA in SLE were also identified that provided a clue to explain the possible regulatory mechanism for the immune function in SLE.ConclusionThe results indicated that the aberrant CD45 isoforms, DNA methylation and hormone levels might be correlated with the imbalanced immune activity of SLE patients.Key Points•Selective immunoglobulin A deficiency was significantly higher in SLE than in healthy individuals.•SLE patients had decreased testosterone and increased prolactin in the sera.•An aberrant expression of CD45 isoforms and CD45 DNA methylation were identified in SLE.