Molecular epidemiology of Cryptosporidium spp. in an agricultural area of northern Vietnam: A community survey
Molecular epidemiology of Cryptosporidium spp. in an agricultural area of northern Vietnam: A community survey
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DOI：
10.1016/j.parint.2021.102341
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发表时间：
2021-04-13
影响因子：
1.9
通讯作者：
Yamashiro, Tetsu
中科院分区：
文献类型：
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作者：
Iwashita, Hanako;Takemura, Taichiro;Yamashiro, Tetsu
本研究的目的是调查隐孢子虫感染的发生情况和隐孢子虫传播的可能性。在越南北部的动物和人类之间。通过对某农业区的社区调查，共采集2715份样品，其中人腹泻样品2120份，非腹泻样品471份，动物粪便样品124份。所有样本都进行了隐孢子虫检测。使用荧光显微镜进行直接免疫荧光分析(DFA)。DNA提取、3个基因(cop、SSU-rRNA和gp60)的PCR扩增和测序分析对隐孢子虫进行鉴定。在2715份样本中，有15份样本(10份腹泻样本、2份非腹泻样本和3份动物粪便样本)经聚合酶链式反应检测呈阳性。三种隐孢子虫。通过对扩增的cop和/或SSUrRNA基因进行测序，鉴定为犬钩端螺旋体(从6个人腹泻样本、2个人类非腹泻样本和1个狗样本)、人钩端螺旋体(从4个人腹泻样本)和猪钩虫(从2个猪样本)。在人形毛滴虫方面，在所有四个人腹泻样本中都检测到了GP60亚型IeA12G3T3。虽然阳性样本的数量很少，但我们的流行病学数据显示，在这个研究地点，三个物种(犬、人和猪)的出现模式是不同的。人毛滴虫和猪毛滴虫分别只在人和猪的样本中检测到，犬毛滴虫在狗和人的样本中都检测到。我们怀疑，在这个研究地点，人类感染犬毛虫可能是由于环境被动物和人的粪便污染所致。
The purpose of this study was to investigate the occurrence of Cryptosporidium infection and the potential for transmission of Cryptosporidium spp. between animals and humans in northern Vietnam. A total of 2715 samples (2120 human diarrheal samples, 471 human non-diarrheal samples, and 124 animal stool samples) were collected through our community survey in an agricultural area. All samples were tested for Cryptosporidium spp. by direct immunofluorescence assay (DFA) using a fluorescent microscope. DNA extraction, PCR amplification of three genes (COWP, SSU-rRNA, and GP60), and sequencing analysis were performed to identify Cryptosporidium spp. Of 2715 samples, 15 samples (10 diarrheal samples, 2 non-diarrheal samples, and 3 animal stool samples) tested positive by PCR for the COWP gene. Three species of Cryptosporidium spp. were identified as C. canis (from six human diarrheal samples, two human non-diarrheal samples, and one dog sample), C. hominis (from four human diarrheal samples), and C. suis (from two pig samples) by sequencing the amplified COWP and/or SSUrRNA genes. In terms of C. hominis, the GP60 subtype IeA12G3T3 was detected in all four human diarrheal samples. Although the number of positive samples was very small, our epidemiological data showed that the emerging pattern of each of the three species (C. canis, C. hominis, and C. suis) was different at this study site. While C. hominis and C. suis were only detected in human and pig samples, respectively, C. canis was detected in samples from both dogs and humans. We suspect that C. canis infections in humans at this study site may be due to environmental contamination with animal and human feces.