Immunological Identification and Characterization of the Capsid Scaffold Protein Encoded by UL26.5 of Herpes Simplex Virus Type 2.
Immunological Identification and Characterization of the Capsid Scaffold Protein Encoded by UL26.5 of Herpes Simplex Virus Type 2.
复制标题
2型单纯疱疹病毒UL26.5编码的衣壳支架蛋白的免疫学鉴定和表征
DOI：
10.3389/fcimb.2021.649722
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发表时间：
2021
影响因子：
5.7
通讯作者：
Li Q
中科院分区：
文献类型：
--
作者：
Li X;Wang J;Mou T;Gao Y;Wang L;Fan S;Xu X;Jiang G;Cui P;Xu X;Duan S;Zhang J;Li D;Liao Y;Yu L;Zhao H;Lu M;Zhu H;Gu R;Zhang Y;Dong W;Li Q
单纯疱疹病毒2型(HSV2)是一种引起生殖器疱疹病变的病原体，通过各种已知和未知的机制干扰宿主免疫系统。该病毒已被用于研究病毒的抗原组成。以HSV2感染者恢复期血清为模板，通过双向蛋白电泳法和免疫印迹法鉴定病毒抗原。血清主要识别UL26.5基因编码的几种衣壳支架蛋白，主要是ICP35。据报道，这种蛋白主要在病毒组装中暂时发挥作用，但在成熟的病毒颗粒中不表达。进一步的免疫学研究表明，该蛋白可在小鼠中引起特异性抗体和细胞毒性T淋巴细胞(CTL)反应，但这些反应不能对HSV2攻击产生临床保护作用。提示ICP35的免疫优势可用于设计与其他病毒糖蛋白整合的抗原。
Herpes simplex virus type 2 (HSV2), a pathogen that causes genital herpes lesions, interferes with the host immune system via various known and unknown mechanisms. This virus has been used to study viral antigenic composition. Convalescent serum from HSV2-infected patients was used to identify viral antigens via 2-D protein electrophoresis and immunoblotting. The serum predominantly recognized several capsid scaffold proteins encoded by gene UL26.5, mainly ICP35. This protein has been primarily reported to function temporarily in viral assembly but is not expressed in mature virus particles. Further immunological studies suggested that this protein elicits specific antibody and cytotoxic T lymphocyte (CTL) responses in mice, but these responses do not result in a clinical protective effect in response to HSV2 challenge. The data suggested that immunodominance of ICP35 might be used to design an integrated antigen with other viral glycoproteins.