STIMULATION-INDUCED UPTAKE AND RELEASE OF ZINC IN HIPPOCAMPAL SLICES
STIMULATION-INDUCED UPTAKE AND RELEASE OF ZINC IN HIPPOCAMPAL SLICES
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DOI：
10.1038/308736a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
FREDERICKSON, CJ
中科院分区：
文献类型：
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作者：
HOWELL, GA;WELCH, MG;FREDERICKSON, CJ
海马体苔藓纤维轴突形成致密的神经丛，特别富含可螯合的锌1-3。由于金属明显集中在轴突4，5的终袋内，因此推测锌参与苔藓纤维突触的传递6，7。尽管一些电生理学发现8，9支持这一假说，但先前还没有发现锌优先摄取到海马区10，11或去极化诱导从海马区组织释放锌。使用海马片制备，我们现在报告苔藓纤维神经桩和起源细胞(齿状颗粒细胞)优先摄取锌，电刺激选择性地促进外源性锌被苔藓纤维神经桩摄取和先前结合的65锌从组织中释放。结果表明，锌在苔藓纤维轴突中的作用与神经信号传递过程动态相关。
The mossy-fibre axons of the hippocampus form a dense plexus, uniquely rich in chelatable zinc1–3. Because the metal is apparently concentrated within the terminal bags of the axons4,5, it has been hypothesized that the zinc is involved in mossy-fibre synaptic transmission6,7. Although some electrophysiological findings8,9have favoured the hypothesis, neither preferential uptake of zinc into the hippocampus10,11nor depolarization-induced release of zinc from hippocampal tissue12has previously been found. Using the hippocampal slice preparation, we now report that the mossy-fibre neuropil and cells of origin (dentate granule cells) take up zinc preferentially, and that electrical stimulation selectively facilitates both uptake of exogenous zinc into mossy-fibre neuropil and release of previously incorporated65Zn from the tissue. The results suggest that the role of zinc in mossy-fibre axons is dynamically linked to neural signalling processes.