Transcriptome analysis of the sea cucumber (Apostichopus japonicus) with variation in individual growth.
Transcriptome analysis of the sea cucumber (Apostichopus japonicus) with variation in individual growth.
复制标题
海参（Apostichopus japonicus）个体生长差异的转录组分析
DOI：
10.1371/journal.pone.0181471
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Ma Z
中科院分区：
文献类型：
--
作者：
Gao L;He C;Bao X;Tian M;Ma Z
仿刺参（Apostichopus cucumber）是我国重要的经济养殖品种。然而，严重的个体生长变异往往给农民造成经济损失，遗传机制知之甚少。本研究在转录组水平上对海参个体生长差异进行了深入分析。从255861个转录本中组装了118946个unigenes，N50为1700。在所有的unigenes中，约23%被鉴定为与已知数据库至少有一个显著匹配。在所有四对比较中，发现1840个基因表达不同。在生长缓慢的群体中发现了整体代谢低下现象，据此提出了海参个体生长变异中的生长迟缓是一种用来对抗逆境的休眠类型的假说。此外，ECM-受体相互作用和粘着斑等途径在维持细胞和组织的结构和通讯中也有重要作用。此外，在本研究中检测到76645个SSR，765242个SNPs和146886个ins-dels，为今后的遗传作图和选择育种研究提供了广泛的数据。这些研究结果将为深入了解海洋无脊椎动物个体生长差异的分子基础和了解生长过程的生理差异提供重要的参考。
The sea cucumber (Apostichopus japonicus) is an economically important aquaculture species in China. However, the serious individual growth variation often caused financial losses to farmers and the genetic mechanisms are poorly understood. In the present study, the extensively analysis at the transcriptome level for individual growth variation in sea cucumber was carried out. A total of 118946 unigenes were assembled from 255861 transcripts, with N50 of 1700. Of all unigenes, about 23% were identified with at least one significant match to known databases. In all four pair of comparison, 1840 genes were found to be expressed differently. Global hypometabolism was found to be occurred in the slow growing population, based on which the hypothesis was raised that growth retardation in individual growth variation of sea cucumber is one type of dormancy which is used to be against to adverse circumstances. Besides, the pathways such as ECM-receptor interaction and focal adhesion were enriched in the maintenance of cell and tissue structure and communication. Further, 76645 SSRs, 765242 SNPs and 146886 ins-dels were detected in the current study providing an extensive set of data for future studies of genetic mapping and selective breeding. In summary, these results will provides deep insight into the molecular basis of individual growth variation in marine invertebrates, and be valuable for understanding the physiological differences of growth process.