Bat lyssaviruses (Aravan and Khujand) from Central Asia: phylogenetic relationships according to N, P and G gene sequences
Bat lyssaviruses (Aravan and Khujand) from Central Asia: phylogenetic relationships according to N, P and G gene sequences
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DOI：
10.1016/s0168-1702(03)00217-x
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发表时间：
2003-11-01
期刊：
影响因子：
5
通讯作者：
Rupprecht, CE
中科院分区：
文献类型：
--
作者：
Kuzmin, IV;Orciari, LA;Rupprecht, CE
1991年和2001年分别在吉尔吉斯斯坦南部和塔吉克斯坦北部分离到蝙蝠溶血病毒Aravan和Khujand。用抗核衣壳单抗进行的初步研究表明，该病毒与其他溶酶病毒血清型不同。这些数据得到了阿拉万病毒N基因序列的支持。在本研究中，我们对Aravan和Khujand病毒的N、P和G基因进行了全序列测定，并将它们分别与GenBank上提供的其他Lyssav病毒的序列进行了比较。结果表明，根据目前的基因分型方法(N基因核苷酸同源性的数量和加入某些系统发育类群的自助性支持)，每个病毒都应被视为一个新识别的基因型别。不同的系统发育方法和基因组不同部分的比较表明，Khujand病毒主要与基因6型有关，而Aravan病毒一方面与Khujand病毒有关，另一方面与基因4、5和6型有一定的相似性。这些新的溶血病毒对兽医和公共卫生的潜在意义不容小觑。(C)2003爱思唯尔B.V.保留所有权利。
Bat lyssaviruses Aravan and Khujand were isolated in southern Kyrgyzstan in 1991 and in northern Tajikistan in 2001, respectively. Preliminary studies with anti-nucleocapsid monoclonal antibodies suggested that the viruses were distinct from other lyssavirus serotypes. These data were supported by sequencing of the N gene of Aravan virus. In the present study, we sequenced the entire N, P and G genes of both Aravan and Khujand viruses and compared them with respective sequences of other lyssaviruses available from GenBank. The results suggested that each virus should be considered as a newly recognized genotype according to the current approaches for genotype definition (amount of nucleotide identity of the N gene and bootstrap support of joining to certain phylogenetic groups). Use of different phylogenetic methods and comparison of different parts of the genomes generally suggested that Khujand virus was mainly related to genotype 6, while Aravan virus, on the one hand, was related to Khujand virus, and, on the other hand, demonstrated moderate similarity to genotypes 4, 5 and 6. The potential significance of these new lyssaviruses for veterinary and public health should not be underestimated. (C) 2003 Elsevier B.V. All rights reserved.