Prokaryotic abundance and 16S rRNA gene sequences detected in marine aerosols on the East Sea (Korea)
Prokaryotic abundance and 16S rRNA gene sequences detected in marine aerosols on the East Sea (Korea)
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DOI：
10.1111/j.1574-6941.2011.01053.x
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发表时间：
2011-05-01
影响因子：
4.2
通讯作者：
Hwang, Chung Yeon
中科院分区：
文献类型：
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作者：
Cho, Byung Cheol;Hwang, Chung Yeon
现代研究海洋空气中的原核生物在公海上已经很少。在这里，了解它们的分布和组成，气溶胶以及表层海水样品收集在东海的三个站点。空气中原核生物的丰度为0.7-1.2 x 105个细胞m-3。气溶胶中16 S rRNA基因的部分序列以γ蛋白菌纲和拟杆菌纲为主。有趣的是，在沿海和偏远的近海地区，空气中细菌的数量与海水细菌的数量相当。在这些气溶胶中检测到的细菌操作分类单位（OTU）中有一半以上（20个中有12个）来自海洋。在地表水中发现的19种细菌OTU中，有8种出现在气溶胶中。沃茨。因此，表层海水似乎是海洋空气细菌的主要来源。然而，在中间的离岸站点，空气中的细菌OTU（n=4）都是陆地的，这表明在1-4天间隔采样的站点（每个站点相距160 km）上发生了主要空气中细菌OTU类型的变化。Euryarchaeota序列属于MSP 8进化枝报告从地理上遥远的地区被检测到海洋气溶胶。海洋原核生物的远距离空中运输似乎是完全可能的。
Modern studies on marine airborne prokaryotes over open seas have been rare. Here, to understand their distribution and composition, aerosol as well as surface seawater samples were collected at three sites in the East Sea. The abundance of airborne prokaryotes was 0.7-1.2 x 105 cells m-3. Partial sequences of the 16S rRNA gene from the aerosols were dominated by Gammaproteobacteria and Bacteroidetes. Interestingly, the numbers of phylotypes of airborne bacteria were comparable to those of seawater bacteria in coastal and remote offshore sites. Over half of the bacterial operational taxonomic units (OTUs) detected in these aerosols (12 out of 20) were of marine origin. Eight of the 19 bacterial OTUs found in the surface waters occurred in the aerosols. Thus, surface seawater seems to be a main source of marine airborne bacteria. However, in the intermediate offshore site, airborne bacterial OTUs (n=4) were all terrestrial, indicating that variations of dominant airborne bacterial phylotypes occurred over the sites (each similar to 160 km apart) sampled at 1-4-day intervals. Euryarchaeota sequences belonging to the MSP8 clade reported from geographically distant regions were detected in marine aerosols. Long-distance aerial transport of the marine prokaryotes seems to be quite possible.