B cells solicit their own help from T cells.
B cells solicit their own help from T cells.
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B细胞从T细胞中寻求自己的帮助。
DOI：
10.1084/jem.183.3.891
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发表时间：
1996-03-01
期刊：
影响因子：
--
通讯作者：
Gray D
中科院分区：
文献类型：
--
作者：
Stockinger B;Zal T;Zal A;Gray D
我们利用T细胞受体（TCR）转基因小鼠CD4+ T细胞表达自身抗原C5（补体第五组分）特异性受体，研究不同抗原呈递细胞在CD4+ T细胞效应类型测定中的作用。体内或体外，C5 tcr转基因小鼠的T细胞与C5蛋白或C5肽接触，可诱导偏倚的辅助性T细胞（Th） 1型反应，导致高水平的干扰素γ和白细胞介素（IL） 2的产生。与非转基因小鼠相比，转基因小鼠不会产生针对C5的抗体反应。我们在本文中表明，B细胞在体外的呈递诱导了Th2亚群的转换，这是由IL-4的产生所指示的，并且在体内将C5靶向B细胞导致C5特异性抗体的产生。
We have made use of T cell receptor (TCR)-transgenic mice with CD4+ T cells expressing a receptor specific for the self-antigen C5 (fifth component of complement) to study the role of different antigen- presenting cells in the determination of CD4+ T cell effector type. Contact of T cells from C5 TCR-transgenic mice with C5 protein or C5 peptide in vivo or in vitro induces biased T helper cell (Th) 1 type responses resulting in exclusive production of high levels of interferon gamma and interleukin (IL) 2. Transgenic mice, in contrast to nontransgenic littermates, do not generate an antibody response to C5. We show in this paper that B cell presentation in vitro induces a switch to the Th2 subset indicated by production of IL-4, and targetting C5 to B cells in vivo results in the generation of C5- specific antibodies.