SCID mouse model for lethal Q fever
SCID mouse model for lethal Q fever
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DOI：
10.1128/iai.71.8.4717-4723.2003
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发表时间：
2003-08-01
影响因子：
3.1
通讯作者：
Hirai, K
中科院分区：
文献类型：
--
作者：
Andoh, M;Naganawa, T;Hirai, K
Q 热是一种由伯内特立克次体引起的世界性人畜共患病，在人类中有多种表现。心内膜炎是Q热最严重的并发症。动物模型仅限于急性肺病或肝病以及生殖障碍。目前尚不存在合适的 Q 热心内膜炎实验动物模型。在这项研究中，感染伯尼衣原体的严重联合免疫缺陷（SCID）小鼠表现出持续的临床症状并死亡，而类似感染的免疫功能正常的小鼠则无症状并存活下来。本研究中检查的 SCID 小鼠的主要器官（心脏、肺、脾、肝和肾）存在严重的慢性损伤。 SCID 小鼠的心脏病变与患有慢性 Q 热心内膜炎的人类相似：它们有局灶性钙化和含有伯尼梭菌的巨噬细胞扩张。 SCID小鼠中C.burni的50%致死剂量比免疫活性小鼠至少低10(8)倍。 SCID小鼠对布尔尼衣原体高度敏感，宿主的免疫缺陷加剧了Q热的严重程度。该动物模型可为研究慢性Q热和免疫缺陷宿主Q热提供新工具。
Q fever, a worldwide zoonosis caused by Coxiella burnetii, has many manifestations in humans. Endocarditis is the most serious complication of Q fever. Animal models are limited to acute pulmonary or hepatic disease and reproductive disorders. An appropriate experimental animal model for Q fever endocarditis does not yet exist. In this study, severe combined immunodeficient (SCID) mice infected with C. burnedi showed persistent clinical symptoms and died, whereas immunocompetent mice similarly infected became asymptomatic and survived. The SCID mice examined in this study had severe chronic lesions in their primary organs: the heart, lung, spleen, liver, and kidney. The heart lesions of the SCID mice were similar to those in humans with chronic Q fever endocarditis: they had focal calcification and expanded macrophages containing C. burnedi. The 50% lethal dose of C. burnedi in SCID mice was at least 10(8) times less than that in immunocompetent mice. The SCID mouse is highly susceptible to C. burnedi, and the immunodeficiency of the host enhances the severity of Q fever. This animal model could provide a new tool for the study of chronic Q fever and Q fever in immunodeficient hosts.