Paraoxonase (PON1) activity in patients with subclinical thoracic aortic atherosclerosis
Paraoxonase (PON1) activity in patients with subclinical thoracic aortic atherosclerosis
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DOI：
10.1007/s10554-014-0407-y
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发表时间：
2014-06-01
影响因子：
2.1
通讯作者：
Aksoy, Nurten
中科院分区：
文献类型：
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作者：
Gur, Mustafa;Cayli, Murat;Aksoy, Nurten
高密度脂蛋白（HDL）是一种强大的抗氧化剂，可保护低密度脂蛋白（LDL）颗粒免受氧化应激。通过限制LDL氧化，HDL在预防动脉粥样硬化（AS）中起重要作用。HDL的抗氧化作用主要与对氧磷酶（PON 1）活性有关。已知主动脉内膜中层厚度（IMT）增加是比颈动脉IMT更早的AS标志物。我们的目的是研究胸主动脉IMT和血清PON 1活性之间的关系。我们研究了133例患者（平均年龄：46.3 +/-A8岁），这些患者因各种适应症接受了经食管超声心动图（TEE）。TEE测量胸主动脉IMT分为1、2、3、4级。酶联免疫吸附法测定血清PON 1活性。通过测定血清脂质过氧化氢（LOOH）、总抗氧化状态（TAS）评价氧化和抗氧化状态。血清PON 1活性从IMT 1级到IMT 4级逐渐降低（p < 0.001）。然而，与其他分级相比，1级患者的血清LOOH显著较低，TAS显著较高。在多元线性回归分析中，IMT与PON 1活性（β =-0.495，p < 0.001）、TAS水平（β =-196，p < 0.009）、年龄（β = 0.145，p = 0.029）和LDL胆固醇水平（β = 0.169，p = 0.009）独立相关。PON 1活性降低与胸主动脉狭窄程度独立相关。除年龄、TAS和LDL胆固醇水平外，PON 1活性可能在胸主动脉粥样硬化的发病中起一定作用。
High density lipoprotein (HDL), a powerful antioxidant, protects low density lipoprotein (LDL) particles against oxidative stress. By limiting LDL oxidation, HDL plays an important role in preventing atherosclerosis (AS). The antioxidant effect of HDL is mostly associated with the paraoxonase (PON1) activity. It has been known that increased aortic intima-media thickness (IMT) is an earlier marker AS than carotid IMT. We aimed to investigate the association between thoracic aortic IMT and serum PON1 activity. We studied 133 patients (mean age: 46.3 +/- A 8 years) who underwent transesophageal echocardiography (TEE) for various indications. The measurements of thoracic aortic IMT by TEE are classified into four grades (1, 2, 3 and 4). Serum PON1 activity was measured spectrophotometrically. Oxidative and anti-oxidative status was evaluated by measuring serum lipid hydroperoxide (LOOH), total anti-oxidant status (TAS). Serum PON1 activity was progressively decreasing from grade 1 IMT to grade 4 IMT (p < 0.001). However, serum LOOH was significantly lower and TAS was significantly higher in patients with grade 1 when compared with other grades. In multiple linear regression analysis, IMT was independently correlated with PON1 activity (beta = -0.495, p < 0.001), TAS level (beta = -196, p < 0.009), age (beta = 0.145, p = 0.029) and LDL cholesterol level (beta = 0.169, p = 0.009). Decreased PON1 activity was independently associated with the extent of thoracic AS. PON1 activity may play a role in pathogenesis of thoracic AS besides age, TAS and LDL cholesterol levels.