Induction of sulfadiazine resistance in vitro in Toxoplasma gondii
Induction of sulfadiazine resistance in vitro in Toxoplasma gondii
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DOI：
10.1016/j.exppara.2012.11.019
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发表时间：
2013-02-01
影响因子：
2.1
通讯作者：
Villena, I.
中科院分区：
文献类型：
--
作者：
Doliwa, C.;Escotte-Binet, S.;Villena, I.
我们通过药物压力在体外诱导刚地弓形虫的两个磺胺嘧啶敏感株，即RH（I型）和ME - 49（II型）产生磺胺嘧啶抗性。首先，利用酶联免疫吸附测定（ELISA）在Vero细胞上评估了刚地弓形虫的两个敏感株和两个天然抗性株对磺胺嘧啶的敏感性。磺胺嘧啶对RH株的半数抑制浓度（IC50）值为77微克/毫升，对ME - 49株为51微克/毫升，对两个天然抗性株则高于1000微克/毫升。其次，通过逐渐增加磺胺嘧啶浓度来诱导菌株产生抗性，经此测试验证，其结果是IC50值高于1000微克/毫升。总之，我们在体外培育出了两个磺胺嘧啶抗性株，称为RH - R - SDZ和ME - 49 - R - SDZ。这些对磺胺嘧啶有抗性的菌株将有助于表征对磺胺嘧啶的抗性机制。（C）2012爱思唯尔公司。保留所有权利。
We induced sulfadiazine resistance in two sulfadiazine sensitive strains of Toxoplasma gondii, RH (Type I) and ME-49 (Type II) in vitro by using drug pressure. At first, sulfadiazine susceptibility of the two sensitive strains and two naturally resistant strains of T. gondii was evaluated on Vero cells using an enzyme-linked immunosorbent assay (ELISA). The IC50 values of sulfadiazine were 77 mu g/mL for RH, 51 mu g/rnL for ME-49 and higher than 1000 mu g/mL for the two natural resistant strains. Secondly, induced resistance of the strains by gradually increase sulfadiazine concentration was verified by this test, which resulted IC50 values at higher than 1000 mu g/mL. In conclusion we developed in vitro two sulfadiazine resistant strains called RH-R-SDZ and ME-49-R-SDZ. These strains resistant to sulfadiazine would be useful to characterize resistance mechanisms to sulfadiazine. (C) 2012 Elsevier Inc. All rights reserved.