Long non-coding RNA Fer-1-like family member 4 is overexpressed in human glioblastoma and regulates the tumorigenicity of glioma cells
Long non-coding RNA Fer-1-like family member 4 is overexpressed in human glioblastoma and regulates the tumorigenicity of glioma cells
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长非编码 RNA Fer-1 样家族成员 4 在人胶质母细胞瘤中过表达并调节胶质瘤细胞的致瘤性
DOI：
10.3892/ol.2017.6403
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发表时间：
2017-08-01
期刊：
影响因子：
2.9
通讯作者：
Xia, Liang
中科院分区：
文献类型：
--
作者：
Ding, Feng;Tang, Hongtu;Xia, Liang
长链非编码RNA（lncRNA）是一类长度大于200个核苷酸的调节性非编码RNA。以往的研究表明，lncRNA Fer-1-like family member 4（FER 1 L4）在肿瘤进展中起调节作用;然而，其在人类神经胶质瘤中的临床意义尚不清楚。在本研究中，从癌症基因组图谱中挖掘数据，以研究FER 1 L4表达与胶质瘤患者预后之间的关系。将靶向FER 1 L4的短干扰RNA转染到U373-MG和U251胶质瘤细胞系中，分别用CCK-8、Transwell和Annexin V-异硫氰酸荧光素/碘化丙啶检测细胞活力、侵袭和凋亡。与低级别胶质瘤相比，FER 1 L4在高级别胶质瘤中显著上调。此外，FER 1 L4的高表达显著预测胶质瘤患者的不良预后。FER 1 L4在胶质瘤细胞系中的表达明显高于正常星形胶质细胞。此外，通过使用siRNA下调FER 1 L4，胶质瘤细胞的侵袭力和存活力显著降低，而凋亡显著增加。本研究结果表明FER 1 L4在胶质瘤的发生和发展中起作用，可作为胶质瘤的预后生物标志物。
Long non-coding RNA (lncRNA) is a class of regulative non-coding RNA that is >200 nucleotides in length. Previous studies have demonstrated that lncRNA Fer-1-like family member 4 (FER1L4) serves regulatory roles in tumor progression; however, its clinical significance in human neuroglioma remains unclear. In the present study, data from The Cancer Genome Atlas was mined in order to investigate the association between FER1L4 expression and prognosis in patients with glioma. A short interfering (si) RNA targeting FER1L4 was transfected into U373-MG and U251 glioma cell lines, and cell viability, invasion and apoptosis were examined using CCK-8, Transwell and Annexin V-fluorescein isothiocyanate/propidium iodide assays, respectively. FER1L4 was significantly upregulated in high-grade glioma compared with low-grade glioma. Additionally, high expression of FER1L4 significantly predicted poor prognosis in patients with glioma. The expression of FER1L4 in glioma cell lines was significantly higher compared with that in normal astrocytes. Furthermore, by downregulating FER1L4 using siRNA, the invasiveness and viability of the glioma cells significantly decreased, while apoptosis significantly increased. The findings from the present study indicate that FER1L4 serves a role in the occurrence and progression of glioma, and could be used as a prognostic biomarker for this disease.