Proline-rich antimicrobial peptide, PR-39 gene transduction altered invasive activity and actin structure in human hepatocellular carcinoma cells.
Proline-rich antimicrobial peptide, PR-39 gene transduction altered invasive activity and actin structure in human hepatocellular carcinoma cells.
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富含脯氨酸的抗微生物肽，PR-39基因转导改变了人肝细胞癌细胞中的侵入性活性和肌动蛋白结构。
DOI：
10.1038/sj.bjc.6690707
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发表时间：
1999-10
影响因子：
8.8
通讯作者：
Kohgo, Y
中科院分区：
文献类型：
--
作者：
Ohtake, T;Fujimoto, Y;Ikuta, K;Saito, H;Ohhira, M;Ono, M;Kohgo, Y
PR-39 是一种富含脯氨酸的内源性抗菌肽，可诱导 syndecan-1 的合成，这是一种参与细胞与基质相互作用和伤口愈合的跨膜硫酸乙酰肝素蛋白聚糖。此前，我们发现syndecan-1在具有高转移潜能的人肝细胞癌中表达降低，推测syndecan-1在抑制侵袭和转移中发挥重要作用。据推测，用 PR-39 和 syndecan-1 对该过程进行修改可能会产生一种抑制侵袭和转移的新策略。因此，我们将PR-39基因转入人肝癌细胞系HLF中，该细胞系显示syndecan-1的低表达和高的体外侵袭活性，并检验该过程是否可以降低肿瘤细胞的侵袭活性。在两个带有PR-39基因的转染子中，syndecan-1表达被诱导，并且I型胶原包被室中的侵袭活性被抑制。此外，除了通过共焦成像系统观察到的肌动蛋白丝的瓦解之外，这些转染子还显示出通过吞噬动力学轨迹测定的运动活性的抑制。相比之下，HLF 细胞中带有 syndecan-1 基因的 5 种转染子显示出侵袭活性受到抑制，但没有改变细胞的运动活性和肌动蛋白结构。这些结果表明，除了抑制可能由于诱导 syndecan-1 表达而导致的侵袭活性外，PR-39 还具有抑制人肝细胞癌细胞运动活性和改变肌动蛋白结构的功能。 © 1999 癌症研究运动
PR-39 is an endogenous proline-rich antimicrobial peptide which induces the synthesis of syndecan-1, a transmembrane heparan sulphate proteoglycan involved in cell-to-matrix interactions and wound healing. Previously, we revealed that the expression of syndecan-1 was reduced in human hepatocellular carcinomas with high metastatic potential and speculated that syndecan-1 played an important role in inhibition of invasion and metastasis. It is assumed that a modification of this process with PR-39 and syndecan-1 may result in a new strategy by which it can inhibit the invasion and metastasis. Therefore, we transduced a gene of PR-39 into human hepatocellular carcinoma cell line HLF, which shows a low expression of syndecan-1 and a high in vitro invasive activity, and examined whether this procedure could reduce the invasive activity of tumour cells. In two transfectants with PR-39 gene, the syndecan-1 expression was induced and the invasive activity in type I collagen-coated chamber was inhibited. Moreover, these transfectants showed the suppression of motile activity assayed by phagokinetic tracks in addition to the disorganization of actin filaments observed by a confocal imaging system. In contrast, five transfectants with syndecan-1 gene in the HLF cells revealed suppression of invasive activity but did not alter the motile activity and actin structures of the cell. These results suggest that PR-39 has functions involved in the suppression of motile activity and alteration of actin structure on human hepatocellular carcinoma cells in addition to the suppression of invasive activity which might result from the induction of syndecan-1 expression. © 1999 Cancer Research Campaign
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DOI：
10.1083/jcb.106.2.423
发表时间：
1988-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Saunders S;Bernfield M
通讯作者：
Bernfield M
DOI：
10.1073/pnas.91.23.11035
发表时间：
1994-11-08
影响因子：
11.1
作者：
GALLO, RL;ONO, M;BERNFIELD, M
通讯作者：
BERNFIELD, M
DOI：
10.1073/pnas.85.24.9562
发表时间：
1988-12-01
影响因子：
11.1
作者：
SANDERSON, RD;BERNFIELD, M
通讯作者：
BERNFIELD, M
影响因子：
2.9
作者：
AYCOCK, RS;SEYER, JM
通讯作者：
SEYER, JM
DOI：
10.1083/jcb.108.4.1547
发表时间：
1989-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Saunders S;Jalkanen M;O'Farrell S;Bernfield M
通讯作者：
Bernfield M