Multiparametric microsensor chips for screening applications
Multiparametric microsensor chips for screening applications
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DOI：
10.1007/s002160000628
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发表时间：
2001-01-01
期刊：
影响因子：
--
通讯作者：
Wolf, B
中科院分区：
文献类型：
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作者：
Ehret, R;Baumann, W;Wolf, B
通过基因数据库和表达研究，可以大大加快药物筛选的药物靶点的确定。从不断增长的文库中鉴定先导化合物是通过高通量筛选平台实现的，因此，为了优化和验证所鉴定的先导化合物，必须对其进行功能研究，而这些功能测试是一个限制因素。在将候选药物用于进一步的耗时和昂贵的阶段之前，例如在动物模型中，通过体外细胞筛选对已鉴定的化合物进行严格的预选是必要的。我们的努力集中在不同微电子传感器的并行开发、适应和集成到微型生物芯片中，用于生理环境中活细胞的多参数、功能在线分析。为了药物筛选的高级阶段和节省动物试验，需要并行和在线获取与不同细胞靶点相关的数据。
The identification of drug targets for pharmaceutical screening can be greatly accelerated by gene databases and expression studies. The identification of leading compounds from growing libraries is realized by high throughput screening platforms.Subsequently, for optimization and validation of identified leading compounds studies of their functionality have to be carried out, and just these functionality tests are a limiting factor. A rigorous preselection of identified compounds by in vitro cellular screening is necessary prior to using the drug candidates for the further time consuming and expensive stage, e.g. in animal models.Our efforts are focused to the parallel development, adaptation and integration of different microelectronic sensors into miniaturized biochips for a multiparametric, functional on-line analysis of living cells in physiologically environments. Parallel and on-line acquisition of data related to different cellular targets is required for advanced stages of drug screening and for economizing animal tests.