Susceptibility of in vitro stimulated PBMC to infection with NSIHIV-1 is associated with levels of CCR5 expression and β-chemokine production

Susceptibility of in vitro stimulated PBMC to infection with NSIHIV-1 is associated with levels of CCR5 expression and β-chemokine production
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DOI：
10.1006/viro.1999.0111
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发表时间：
2000-02-15
期刊：
VIROLOGY
影响因子：
3.7
通讯作者：
Schuitemaker, H
Schuitemaker, H
中科院分区：
医学3区
文献类型：
--
作者：
Blaak, H;Ran, LJ;Schuitemaker, H

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分析了14名健康献血员经PHA/rIL-2刺激的PBMC对NSI HIV-1感染的易感性与CCR 5表达和β-趋化因子产生的关系。在rIL-2存在下培养1周后，但不是在接种时，CCR 5表面表达呈阳性，β-趋化因子的产生与NSI HIV-I感染的易感性呈负相关。令人惊讶的是，在CCR 5基因型和体外NSI HIV-1易感性之间没有观察到关联，这与PHA/rIL-2刺激后CCR 5 Delta 32/+和CCR 5 +/+ PBMC中相似水平的CCR 5表面表达和β-趋化因子产生一致。与在体外观察到的相反，CCR 5基因型确实与体内静息以及活化的CD 4（+）T细胞群体中的CCR 5表面表达水平相关，所述活化的CD 4（+）T细胞群体通过CD 45 RO、CD 27、HLA-DR和CD 69的表达来鉴定。体外观察到的CCR 5表达与NSI HIV-1感染易感性之间的相关性可能为体内观察到的影响CCR 5表达的CCR 5多态性对疾病进展的保护作用提供了解释。(C)北京大学出版社.
Susceptibility of PHA/rIL-2-stimulated PBMC from 14 healthy blood donors for NSI HIV-1 infection was analyzed in relation to CCR5 expression and beta-chemokine production. After 1 week of culture in the presence of rIL-2, but not at the moment of inoculation, CCR5 surface expression was positively and beta-chemokine production was inversely associated with susceptibility to NSI HIV-I infection. Surprisingly, no association was observed between CCR5 genotype and in vitro NSI HIV-1 susceptibility, which was in agreement with similar levels of CCR5 surface expression and beta-chemokine production in CCR5 Delta 32/+ and CCR5 +/+ PBMC after PHA/rIL-2 stimulation. In contrast to what was observed in vitro, CCR5 genotype did associate with CCR5 surface expression levels in vivo in resting as well as in activated CD4(+) T cell populations that were identified by the expression of CD45RO, CD27, HLA-DR, and CD69. The association between CCR5 expression and susceptibility to infection by NSI HIV-1 observed in vitro might offer an explanation for the in vivo observed protective effect of CCR5 polymorphisms that influence CCR5 expression on disease progression. (C) 2000 Academic Press.