METHODS FOR DETERMINING PEROXIDE IN LIPIDS
METHODS FOR DETERMINING PEROXIDE IN LIPIDS
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DOI：
10.1002/jsfa.2740031205
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发表时间：
1952-01-01
影响因子：
4.1
通讯作者：
LEA, CH
中科院分区：
文献类型：
--
作者：
LEA, CH
使用油酸甲酯、亚油酸酯和亚麻酸酯在标准条件下氧化至已知的氧饱和度，比较了测定脂肪材料中过氧化物氧的三种方法的相对效率。碘量法表明71-91%的吸收氧转化为过氧化合物，但硫氰酸铁和2：6-二氯酚靛酚法在有大气氧存在的情况下得到异常高的结果，而在没有大气氧的情况下得到的值明显过低。然而，需氧硫氰酸铁法显示出良好的重现性，需要的材料比碘量法少得多，2：6-二氯酚靛酚法在大多数方面与硫氰酸铁法相似，但重现性较差，而且受大气中氧气和痕量铜的干扰更明显。
The relative efficiencies of three methods for the determination of peroxide oxygen in fatty materials have been compared using methyl oleate, linoleate and linolenate oxidized under standard conditions to known oxygen‐absorptions. The iodometric method indicated the conversion of 71–91% of the oxygen absorbed to peroxy‐compounds, but the ferric thiocyanate and 2: 6‐dichlorophenolindophenol methods both gave abnormally high results in the presence of atmospheric oxygen, and values which were obviously too low in its absence.Nevertheless, the aerobic ferric thiocyanate method shows excellent reproducibility, requires much less material than the iodometric method, and under any one set of conditions gives results which appear to be directly proportional to the iodometric values.The 2: 6‐dichlorophenolindophenol method behaved similarly in most respects to the ferric thiocyanate method, but reproducibility appeared to be poorer and interference by atmospheric oxygen and by traces of copper when present was more marked.