Collecting data in the home laboratory: evolution of X-ray sources, detectors and working practices
Collecting data in the home laboratory: evolution of X-ray sources, detectors and working practices
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DOI：
10.1107/s0907444913013619
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发表时间：
2013-07-01
期刊：
影响因子：
--
通讯作者：
Skarzynski, Tadeusz
中科院分区：
文献类型：
--
作者：
Skarzynski, Tadeusz
虽然目前大多数大分子X射线数据是在越来越多的同步加速器上使用高效光束线收集的，但仍然需要高性能可靠的系统用于内部实验。除了在同步加速器行程之前进行晶体筛选和数据收集参数的优化之外，家庭系统还允许在晶体生产后立即收集数据，以获得新结构的解决方案，特别是通过分子置换方法，并且在实现制药行业中配体结合研究所需的快速周转方面非常宝贵。近年来，为内部使用而开发的X射线源、探测器和软件不断发展，并为结构生物学实验室提供了各种各样的工具。本文概述了这些发展的主要方向和具体的解决方案提供给大分子晶体学社区的例子，显示数据收集“在家里”仍然是一个有吸引力的主张，补充使用同步加速器光束线。
While the majority of macromolecular X-ray data are currently collected using highly efficient beamlines at an ever-increasing number of synchrotrons, there is still a need for high-performance reliable systems for in-house experiments. In addition to crystal screening and optimization of data-collection parameters before a synchrotron trip, the home system allows the collection of data as soon as the crystals are produced to obtain the solution of novel structures, especially by the molecular-replacement method, and is invaluable in achieving the quick turnover that is often required for ligand-binding studies in the pharmaceutical industry. There has been a continuous evolution of X-ray sources, detectors and software developed for in-house use in recent years and a diverse range of tools for structural biology laboratories are available. An overview of the main directions of these developments and examples of specific solutions available to the macromolecular crystallography community are presented in this paper, showing that data collection 'at home' is still an attractive proposition complementing the use of synchrotron beamlines.