Optical probing of acetylcholine receptors on neurons in the medial habenula with a novel caged nicotine drug analogue
Optical probing of acetylcholine receptors on neurons in the medial habenula with a novel caged nicotine drug analogue
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DOI：
10.1113/jp276615
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发表时间：
2018-10
期刊：
影响因子：
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通讯作者：
Stefan Passlick;Ek Raj Thapaliya;Zuxin Chen;Matthew T. Richers;G. Ellis‐Davies
中科院分区：
文献类型：
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作者：
Stefan Passlick;Ek Raj Thapaliya;Zuxin Chen;Matthew T. Richers;G. Ellis‐Davies
开发了一种新的笼状烟碱乙酰胆碱受体（nAChR）激动剂，ABT594，其通过单光子和双光子激发光解。笼状化合物以0.20的量子产率光解。ABT 594的单光子释放在小鼠脑切片的内侧缰（MHb）神经元的索马和树突处引起大电流和Ca2+瞬变。出乎意料的是，ABT 594的解开也揭示了MHb神经元轴突上的高Ca2+渗透性nAChR。
A new caged nicotinic acetylcholine receptor (nAChR) agonist was developed, ABT594, which is photolysed by one‐ and two‐photon excitation. The caged compound is photolysed with a quantum yield of 0.20. One‐photon uncaging of ABT594 elicited large currents and Ca2+ transients at the soma and dendrites of medial habenula (MHb) neurons of mouse brain slices. Unexpectedly, uncaging of ABT594 also revealed highly Ca2+‐permeable nAChRs on axons of MHb neurons.