Transcriptional signature of accessory cells in the lateral line, using the Tnk1bp1:EGFP transgenic zebrafish line.
Transcriptional signature of accessory cells in the lateral line, using the Tnk1bp1:EGFP transgenic zebrafish line.
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DOI：
10.1186/1471-213x-12-6
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发表时间：
2012-01-24
影响因子：
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通讯作者：
Burgess SM
中科院分区：
文献类型：
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作者：
Behra M;Gallardo VE;Bradsher J;Torrado A;Elkahloun A;Idol J;Sheehy J;Zonies S;Xu L;Shaw KM;Satou C;Higashijima S;Weinstein BM;Burgess SM
由于这两个系统之间的结构和分子相似性，侧线，鱼类和两栖动物特有的感觉器官，已被广泛用于斑马鱼作为模型，研究内耳神经上皮细胞的发育/生物学。这两个器官都有毛细胞，这是机械感受器细胞，和支持细胞提供其他功能的上皮。在大多数脊椎动物（不包括哺乳动物）中，支持细胞包括一群替代受损或死亡毛细胞的祖细胞。然而，哺乳动物缺乏再生能力是人类获得性听力障碍的唯一主要原因。为了理解鱼类毛细胞的再生过程，我们鉴定并克隆了一条捕获了tnks 1bp 1的egfp转基因稳定鱼，tnks 1bp 1是一个高度保守的基因，与端粒长度的维持有关。然后，我们在FACsorting策略中使用该Tg（tnks 1bp 1：EGFP）系并结合微阵列来识别支持细胞的新分子标记物。我们提出了一个Tg（tnks 1bp 1：EGFP）稳定的转基因系，我们用来建立支持细胞在斑马鱼侧线的转录谱。因此，我们提供了一组新的特异性支持细胞的标记物，以及用于这种重要细胞类型的功能分析的候选物。这将被证明是一个有价值的工具，特别是在斑马鱼侧线再生的研究和一般的神经上皮细胞的再生。
Because of the structural and molecular similarities between the two systems, the lateral line, a fish and amphibian specific sensory organ, has been widely used in zebrafish as a model to study the development/biology of neuroepithelia of the inner ear. Both organs have hair cells, which are the mechanoreceptor cells, and supporting cells providing other functions to the epithelium. In most vertebrates (excluding mammals), supporting cells comprise a pool of progenitors that replace damaged or dead hair cells. However, the lack of regenerative capacity in mammals is the single leading cause for acquired hearing disorders in humans. In an effort to understand the regenerative process of hair cells in fish, we characterized and cloned an egfp transgenic stable fish line that trapped tnks1bp1, a highly conserved gene that has been implicated in the maintenance of telomeres' length. We then used this Tg(tnks1bp1:EGFP) line in a FACsorting strategy combined with microarrays to identify new molecular markers for supporting cells. We present a Tg(tnks1bp1:EGFP) stable transgenic line, which we used to establish a transcriptional profile of supporting cells in the zebrafish lateral line. Therefore we are providing a new set of markers specific for supporting cells as well as candidates for functional analysis of this important cell type. This will prove to be a valuable tool for the study of regeneration in the lateral line of zebrafish in particular and for regeneration of neuroepithelia in general.