HPAEC-PAD and Q-TOF-MS/MS analysis reveal a novel mode of action of endo-β-1,3(4)-d-glucanase Eng16A from Coprinopsis cinerea on barley β-glucan
HPAEC-PAD and Q-TOF-MS/MS analysis reveal a novel mode of action of endo-β-1,3(4)-d-glucanase Eng16A from Coprinopsis cinerea on barley β-glucan
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HPAEC-PAD 和 Q-TOF-MS/MS 分析揭示了来自灰霉鬼伞 (Coprinopsis cinerea) 的内切β-1,3(4)-d-葡聚糖酶 Eng16A 对大麦β-葡聚糖的新作用模式
DOI：
10.1016/j.foodchem.2019.02.086
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发表时间：
2019
期刊：
影响因子：
8.8
通讯作者：
Sheng Yuan
中科院分区：
文献类型：
--
作者：
Yuanjing Xiong;Yanxin Wang;Maomao Li;Liqin Kang;Jiangsheng Zhou;Cuicui Liu;Zhonghua Liu;Zhenqing Zhang;Sheng Yuan
We previously reported that an endo-β-1,3(4)-d-glucanase, Eng16A, fromC. cinereashows a higher degradation activity toward barley β-glucan than laminarin. HPAEC-PAD and Q-TOF-MS/MS analyses show that Eng16A-digestion products of barley β-glucan not only contain some oligosaccharides with (1 → 3)-β-linkage adjacent to the reducing end, which is consistent with β-1,3(4)-glucanase-digestion products, but also include some oligosaccharides containing (1 → 4)-β-linkage adjacent to the reducing end which is consistent with cellulase-digestion products. Thus, Eng16A possesses both cellulase and β-1,3(4)-glucanase activities. Because Eng16A does not degrade cellulose, we propose that the insertion of a (1 → 3)-β-linkage among the groups of (1 → 4)-β-linkages may make these (1 → 4)-β-linkages prone to cleavage by Eng16A. Furthermore, Eng16A also possesses transglycosylation activity which leads to some products containing one or a few consecutive (1 → 3)-β-linkages adjacent to the non-reducing end. Therefore, HPAEC-PAD and Q-TOF-MS/MS analyses provide an efficient approach to reveal complicated modes of action of some endo-β-1,3(4)-d-glucanases on barley β-glucan.
