Carbon source utilization and inhibitor tolerance of 45 oleaginous yeast species.
Carbon source utilization and inhibitor tolerance of 45 oleaginous yeast species.
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DOI：
10.1007/s10295-014-1447-y
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发表时间：
2014-07
影响因子：
3.4
通讯作者：
Boundy-Mills K
中科院分区：
文献类型：
--
作者：
Sitepu I;Selby T;Lin T;Zhu S;Boundy-Mills K
使用含油（高脂质）酵母将木质纤维素水解产物转化为脂质需要将水解产物组成与酵母菌株的特征相匹配，包括利用某些营养物质的能力、独立于昂贵营养物质（例如维生素）生长的能力以及耐受抑制剂的能力。这些特征的某种组合可能存在于野生菌株中。在这项研究中，测试了属于 45 个物种的 48 种产油酵母菌株利用与木质纤维素水解产物相关的碳源、耐受抑制剂以及在不添加维生素的培养基中生长的能力。一些经过充分研究的产油酵母物种，以及一些尚未在研究或工业生产中经常使用的酵母物种，由于能够利用许多碳源而成为工业用途的有前途的候选者，包括金黄色葡萄球菌、劳伦氏隐球菌、Hanaella aff。玉米、银耳和 Trichosporon coremiiforme。其他物种在抑制剂耐受性方面表现出色，包括念珠菌 aff。 Tropicalis、Cyberlindnera jadinii、Metschnikowia pulcherrima Schwanniomyces occidentalis 和 Wickerhamomyces ciferii。所测试的酵母都无法利用所有碳源并耐受所有测试的抑制剂。这些结果表明，应根据与目标水解产物的组成相容的特性来选择酵母菌株。其他需要考虑的因素包括有价值的副产品（如类胡萝卜素）的生产、遗传工具的可用性、生物安全水平和酵母菌株的絮凝。这项研究产生的数据将有助于将酵母与相容的水解产物结合起来，将碳水化合物转化为脂质，用于生物燃料和其他油脂化学品。
Conversion of lignocellulosic hydrolysates to lipids using oleaginous (high lipid) yeasts requires alignment of the hydrolysate composition with the characteristics of the yeast strain, including ability to utilize certain nutrients, ability to grow independently of costly nutrients such as vitamins, and ability to tolerate inhibitors. Some combination of these characteristics may be present in wild strains. In this study, 48 oleaginous yeast strains belonging to 45 species were tested for ability to utilize carbon sources associated with lignocellulosic hydrolysates, tolerate inhibitors, and grow in medium without supplemented vitamins. Some well-studied oleaginous yeast species, as well as some that have not been frequently utilized in research or industrial production, emerged as promising candidates for industrial use due to ability to utilize many carbon sources, including Cryptococcus aureus, Cryptococcus laurentii, Hanaella aff. zeae, Tremella encephala, and Trichosporon coremiiforme. Other species excelled in inhibitor tolerance, including Candida aff. tropicalis, Cyberlindnera jadinii, Metschnikowia pulcherrima Schwanniomyces occidentalis and Wickerhamomyces ciferii. No yeast tested could utilize all carbon sources and tolerate all inhibitors tested. These results indicate that yeast strains should be selected based on characteristics compatible with the composition of the targeted hydrolysate. Other factors to consider include the production of valuable co-products such as carotenoids, availability of genetic tools, biosafety level, and flocculation of the yeast strain. The data generated in this study will aid in aligning yeasts with compatible hydrolysates for conversion of carbohydrates to lipids to be used for biofuels and other oleochemicals.