Long-term storable and shippable lipid bilayer membrane platform
Long-term storable and shippable lipid bilayer membrane platform
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DOI：
10.1039/b807932c
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发表时间：
2008-10-01
期刊：
影响因子：
6.1
通讯作者：
Schmidt, Jacob J.
中科院分区：
文献类型：
--
作者：
Jeon, Tae-Joon;Poulos, Jason L.;Schmidt, Jacob J.
常规形成的脂质双层膜的易碎性和短寿命特性使得它们的制备必须在使用的时间和地点进行。通过使用高冰点的脂质-溶剂混合物，脂质双分子层的自组装过程可以被可逆地阻止。在固体形式下，双层前体可以无限期储存，并且足够坚固，可以承受商业运输。解冻后，双层自组装恢复，形成具有生物功能的膜。这种膜前驱体与廉价芯片的结合产生了一个紧凑、实用和一次性的离子通道测量平台。
The fragility and short lifetimes characteristic of conventionally formed lipid bilayer membranes has necessitated their preparation to be at the time and point of use. By using high freezing-point lipid-solvent mixtures, the process of lipid bilayer self-assembly may be reversibly arrested. In solid form, the bilayer precursor can be stored indefinitely and is sufficiently robust to withstand commercial shipping. Upon thawing, bilayer self-assembly resumes, resulting in a biologically functional membrane. Combination of this membrane precursor with an inexpensive chip results in a compact, practical, and disposable platform for ion channel measurements.