Multi-centre investigation on reference ranges for ROTEM thromboelastometry
Multi-centre investigation on reference ranges for ROTEM thromboelastometry
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DOI：
10.1097/01.mbc.0000169225.31173.19
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发表时间：
2005-06-01
影响因子：
1.1
通讯作者：
Lakner, M
中科院分区：
文献类型：
--
作者：
Lang, T;Bauters, A;Lakner, M
使用ROTEM全血止血分析仪进行的试剂支持血栓弹性测定（TEM）是血栓弹性描记术的增强，血栓弹性描记术是一种越来越多地用于急性围手术期出血性疾病的床旁监测的方法。我们研究了两个活化试验（INTEM和EXTEM）和一个多中心方法中专门分析凝血纤维蛋白组分（FIBTEM）的试验的参考范围。获得的凝血时间（CT）、血凝块形成时间（CFT）、α角（ALP）、最大血凝块硬度（MCF）和血凝块溶解参数的参考范围在各中心之间相当。Intem：CT = 137-246 s，CFT = 40-100 s，MCF = 52-72 mm。EXTEM：CT = 42-74 s，CFT = 46-148 s，MCF = 49-71 mm。FIBTEM：MCF = 925 mm。与男性相比，女性和老年人中，ROTEM全血凝固与增强凝固趋势弱相关。结果的重复性（运行内不精密度）取决于具有以下变异系数的检测：1-5%（凝块硬度，α角）、3-12%（CT、CFT）、6-13%（FIBTEM凝块硬度）。在抽取后6 h内储存的柠檬酸盐血液样本可稳定用于ROTEM分析。总之，数据表明，ROTEM血栓弹性测定在各中心之间产生一致的值，并且提供一般定向参考范围似乎是可能的。(C)利平科特威廉姆斯&威尔金斯
Reagent-supported thromboelastometry (TEM) with the ROTEM Whole Blood Haemostasis Analyser is an enhancement of thromboelastography, a method that is increasingly used for the point of care monitoring of acute perioperative bleeding disorders. We investigated the reference ranges of two activated tests (INTEM and EXTEM) and a test analysing specifically the fibrin component of coagulation (FIBTEM) in a multi-centre approach. The reference ranges obtained for the clotting time (CT), clot formation time (CFT), alpha angle (ALP), maximum clot firmness (MCF) and clot lysis parameters were comparable from Centre to centre. INTEM: CT = 137-246 s, CFT = 40-100 s, MCF = 52-72 mm. EXTEM: CT = 42-74 s, CFT = 46-148 s, MCF = 49-71 mm. FIBTEM: MCF = 925 mm. ROTEM whole blood coagulation correlated weakly with a trend towards enhanced coagulation in females compared with males and in advanced age. The repeatability (within-run imprecision) of the results was dependent on the test with the following coefficients of variation: 1-5% (clot firmness, alpha angle), 3-12% (CT, CFT), 6-13% (FIBTEM clot firmness). Citrated blood samples were stable for ROTEM analysis stored within 6 h from drawing. In summary, the data showed that ROTEM thromboelastometry yields consistent values between centres and that providing general orientating reference ranges seems to be possible. (C) Lippincott Williams & Wilkins