Homology exists among the transforming sequences of avian and feline sarcoma viruses.
Homology exists among the transforming sequences of avian and feline sarcoma viruses.
复制标题
禽肉瘤病毒和猫肉瘤病毒的转化序列之间存在同源性。
DOI：
10.1073/pnas.77.11.6536
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发表时间：
1980
影响因子：
11.1
通讯作者：
Stephenson,JR
中科院分区：
文献类型：
--
作者：
Shibuya,M;Hanafusa,T;Hanafusa,H;Stephenson,JR
鸡藤并肉瘤病毒（Fujinami sarcoma virus，FSV）不含与劳斯肉瘤病毒src基因相关的核苷酸序列，但携带至少3000个碱基的独特序列，可能编码该病毒的转化蛋白。利用与FSV独特序列互补的放射性DNA，我们研究了FSV与脊椎动物中其他肉瘤白血病逆转录病毒的相关性。在中等严格条件下，FSV cDNA和Rous肉瘤病毒、Y 73禽肉瘤病毒、几种代表性禽急性白血病病毒或Abelson鼠白血病病毒的RNA之间未检测到交叉杂交。然而，该cDNA确实与鸡PRCII肉瘤病毒的RNA杂交达到56%的程度。FSV cDNA与猫肉瘤病毒Gardner-Arnstein株和Snyder-Theilen株的RNA杂交率分别为27%和19%，而与麦克多诺猫肉瘤病毒的RNA杂交率不高。对杂交体热变性的研究表明，与FSV同源杂交体的解链温度相比，FSV cDNA与PRCII和Gardner-Arnstein猫肉瘤病毒RNA的异源双链体的解链温度分别低7 ℃和12 ℃，并且表明两种异源双链体中的错配小于10%。这些结果表明，与至少一部分FSV独特序列密切相关的核苷酸序列存在于鸡和猫中获得的其他肉瘤病毒的基因组中。
Fujinami sarcoma virus (FSV) of chickens does not contain nucleotide sequences related to the src gene of Rous sarcoma virus, but it carries unique sequences of at least 3000 bases, which are likely to code for the transforming protein of this virus. Using radioactive DNA complementary to FSV-unique sequences, we investigated the relatedness of FSV to other sarcoma-leukemia retroviruses in vertebrates. Under conditions of moderate stringency, no cross-hybridization was detected between FSV cDNA and RNAs of Rous sarcoma virus, Y73 avian sarcoma virus, several representative avian acute leukemia viruses, or Abelson murine leukemia virus. This cDNA, however, did hybridize with RNA of PRCII sarcoma virus of chickens to the extent of 56%. In addition, FSV cDNA was found to hybridize with RNAs of Gardner-Arnstein and Snyder-Theilen strains of feline sarcoma virus to the extent of 27% and 19%, respectively, but not with RNA of McDonough feline sarcoma virus. Studies on thermal denaturation of hybrids showed that the melting temperatures of the heteroduplexes of the FSV cDNA with RNAs of PRCII and Gardner-Arnstein feline sarcoma virus were 7 degrees C and 12 degrees C lower, respectively, compared with the melting temperature of the homologous hybrid of FSV, and suggested less than 10% mismatching in both heteroduplexes. These results indicate that nucleotide sequences closely related to at least a part of FSV-unique sequences are present in the genomes of other sarcoma viruses obtained in chickens and in cats.