A vertically integrated nanoscale tipped microprobe intracellular electrode array
A vertically integrated nanoscale tipped microprobe intracellular electrode array
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垂直集成纳米级尖端微探针细胞内电极阵列
DOI：
10.1109/memsys.2014.6765597
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Takeshi Kawano
中科院分区：
文献类型：
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作者：
Yoshihiro Kubota;Hideo Oi;Hirohito Sawahata;Akihiro Goryu,Yoriko Ando;Rika Numano;Makoto Ishida,;Takeshi Kawano
在这里，我们报告集成纳米尖端120 μ m长的垂直微探针电极（NTE）阵列和细胞内记录使用小鼠腓肠肌。NTE的尖端直径和曲率半径分别为< 200 nm，暴露尖端部分的控制高度为4 μm。在1 kHz生理盐水中，所制造的NTE的阻抗为3.1M Ω，输出/输入信号幅度比为50%。穿透到小鼠肌肉中的NTE检测到幅度为10 - 200 mV的静息膜电位，表明NTE设备检测到来自小鼠肌肉的细胞内信号。尽管我们已经证明了使用肌肉的细胞内记录能力，但是这种具有高纵横比的纳米级电极可以用于包括脑切片在内的许多神经元组织内的多位点细胞内记录。
Here we report integration of nanoscale tipped 120-μm-long vertical microprobe electrode (NTE) array and intracellular recordings using a gastrocnemius muscle of a mouse. The tip diameter and curvature radius of the NTE was < 200 nm, respectively, and the controlled height of the exposed tip section was 4 μm. The impedance of the fabricated NTE exhibited 3.1 MΩ at 1 kHz in saline, with the output/input signal amplitude ratio of 50%. The penetrated NTE into the muscle of a mouse detected the resting membrane potentials with the amplitude of ∼ −200 mV, indicating that the NTE device detected intracellular signals from the mouse's muscle. Although we have demonstrated the intracellular recording capability using a muscle, such nanoscale electrodes with a high aspect ratio can be used for multisite intracellular recordings within numerous neuronal tissues including brain slice.