Color-coded fluorescence imaging of tumor-host interactions
Color-coded fluorescence imaging of tumor-host interactions
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DOI：
10.1038/nprot.2006.119
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发表时间：
2006-01-01
期刊：
影响因子：
14.8
通讯作者：
Yang, Meng
中科院分区：
文献类型：
--
作者：
Hoffman, Robert M.;Yang, Meng
荧光蛋白具有非常明亮和高量子产率的特性，并且有多种颜色可供选择。肿瘤-宿主模型包括在几乎所有细胞和组织中表达绿色荧光蛋白(GFP)的转基因小鼠或在血管等特定组织中选择性表达GFP的转基因小鼠。特别有用的是相应的转GFP基因的裸鼠，因为它们可以接受人类肿瘤。当表达红色荧光蛋白的肿瘤细胞被移植到表达GFP的小鼠体内时，可以观察到各种类型的肿瘤-宿主相互作用，包括涉及宿主血管、淋巴细胞、肿瘤相关成纤维细胞、巨噬细胞、树突状细胞等的相互作用。“颜色编码”的肿瘤-宿主模型可以进行成像，从而更深入地了解所涉及的宿主细胞及其在肿瘤进展中的功能。这些程序大约需要4-8周。
Fluorescent proteins have the properties of being very bright with high quantum yield and are available in many colors. Tumor- host models consist of transgenic mice expressing green fluorescent protein ( GFP) in essentially all cells and tissues or expressing GFP selectively in specific tissues such as blood vessels. Particularly useful are the corresponding nude mice transgenic for GFP expression, as they can accept human tumors. When tumor cells expressing red fluorescent protein are implanted in mice expressing GFP, various types of tumor- host interactions can be observed, including those involving host blood vessels, lymphocytes, tumor- associated fibroblasts, macrophages, dendritic cells and others. The 'color- coded' tumor- host models enable imaging and therefore a deeper understanding of the host cells involved and their function in tumor progression. Approximately 4 - 8 weeks are needed for these procedures.