Recent Strategies for the Biosynthesis of Ergothioneine.
Recent Strategies for the Biosynthesis of Ergothioneine.
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DOI：
10.1021/acs.jafc.1c05280
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发表时间：
2021-11
影响因子：
6.1
通讯作者：
Yibin Qiu;Zhonglin Chen;E. Su;Libin Wang;Liang Sun;Peng Lei;Hong Xu;Sha Li
中科院分区：
文献类型：
--
作者：
Yibin Qiu;Zhonglin Chen;E. Su;Libin Wang;Liang Sun;Peng Lei;Hong Xu;Sha Li
麦角硫因 (EGT) 是一种独特的天然氨基酸，通常由细菌和真菌生物合成。作为一种食品来源的抗氧化剂和细胞保护剂，它具有多种生理益处，在食品、医药和化妆品中具有广泛的应用。传统生产EGT主要通过生物提取或化学合成；然而，这些方法效率低下，使得大规模生产难以满足不断增长的市场需求。如今，合成生物学的快速发展极大地加速了微生物发酵生产EGT的研究。本文对EGT的生物学特性、应用、生物合成、分离和检测方法进行了全面综述。此外，还讨论了工程微生物细胞高效合成EGT的方法和挑战。这项工作为EGT生产提供了新的思路和未来的研究潜力。
Ergothioneine (EGT) is a unique naturally occurring amino acid that is usually biosynthesized by bacteria and fungi. As a food-derived antioxidant and cytoprotectant, it has several physiological benefits and has a wide range of applications in food, medicine, and cosmetics. Traditional production of EGT is mainly through biological extraction or chemical synthesis; however, these methods are inefficient, making large-scale production to meet the growing market demand difficult. Nowadays, the rapid development of synthetic biology has greatly accelerated the research on the EGT production by microbial fermentation. In this paper, the biological characteristics, applications, biosynthesis, separation, and detection methods of EGT were fully reviewed. Furthermore, the approaches and challenges for engineering microbial cells to efficiently synthesize EGT were also discussed. This work provides new ideas and future research potentials in EGT production.