Studies of lactate dehydrogenase in the purified state and in intact erythrocytes.
Studies of lactate dehydrogenase in the purified state and in intact erythrocytes.
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研究纯化状态和完整红细胞中的乳酸脱氢酶。
DOI：
10.1042/bj2020581
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
D. Foxall
中科院分区：
文献类型：
--
作者：
R. Simpson;K. Brindle;F. F. Brown;I. Campbell;D. Foxall
用p.m.r观察乳酸和丙酮酸之间的同位素交换，研究了完整红细胞中的乳酸脱氢酶。草酸和丙酮酸对完整细胞中乳酸脱氢酶的抑制作用与纯化后的酶相似。该酶在完整细胞中的活性表明，红细胞中NAD+ + NADH的游离溶液浓度约为10 μ m，而总可提取的NAD+ + NADH约为80 nmol/ml细胞水。
Lactate dehydrogenase in intact erythrocytes was studied by observing isotope exchange between lactate and pyruvate by p.m.r. The inhibition of the enzyme in intact cells by both oxalate and pyruvate was found to be similar to that of the purified enzyme. The activity of the enzyme in intact cells indicates that the free solution NAD+ + NADH concentration in erythrocytes is about 10 microM whereas the total extractable NAD+ + NADH is about 80 nmol/ml of cell water.