Microcystis aeruginosa and M. wesenbergii Were the Primary Planktonic Microcystin Producers in Several Bulgarian Waterbodies (August 2019)
Microcystis aeruginosa and M. wesenbergii Were the Primary Planktonic Microcystin Producers in Several Bulgarian Waterbodies (August 2019)
复制标题
铜绿微囊藻和韦森伯氏微囊藻是保加利亚多个水体中浮游微囊藻毒素的主要生产者（2019 年 8 月）
DOI：
10.3390/app11010357
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
G. Gärtner
中科院分区：
文献类型：
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作者：
Maya P. Stoyneva;K. Stefanova;J. Descy;Blagoy A. Uzunov;M. Radkova;V. Pavlova;M. Mitreva;G. Gärtner
对有害蓝藻水华的日益关注促进了对潜在有毒物种及其有害物质的藻类学和生态学研究的增加。本研究的目的是确定主要的微囊藻毒素（MC）生产者和他们的贡献，在保加利亚浅水水体的浮游植物，采用不同的方法。2019年8月在9个湖泊和水库中进行了采样，其中两个（水库Kriva Reka和Izvornik 2）是首次研究。蓝原核生物的总物种组成和浮游植物丰度的高贡献证明了光学显微镜（LM）的观察和标记色素的HPLC分析。通过PCR扩增mcyE和mcyB基因支持潜在MC生产者的LM鉴定。通过HPLC-DAD检测的MCs量因位点而异，范围从不可检测的浓度到0.46 µg L−1，仅记录了一种变体，即MC-LR。仅在Mandra和Durankulak水库中发现，而通过PCR从另外五个水体中获得了致突变MC菌株。LM和PCR结果均表明，产MC菌为铜绿微囊藻和M. wesenbertus，尽管它们的出现量很低（<0.5-5%的总生物量）时，丝状氰原核生物占主导地位。
The rising interest in harmful cyanoprokaryote blooms promotes an increase of phycological and ecological research on potentially toxic species and their hazardous substances. The present study aimed to identify the main microcystin (MC) producers and their contribution to the phytoplankton of shallow waterbodies in Bulgaria, applying different methods. The sampling was performed in August 2019 in nine lakes and reservoirs, two of which (reservoirs Kriva Reka and Izvornik 2) were studied for the first time. The high contribution of cyanoprokaryotes to the total species composition and phytoplankton abundance was proved by light microscopic (LM) observations and HPLC analysis of marker pigments. The LM identification of potential MC-producers was supported by PCR amplification of mcyE and mcyB genes. The MCs amounts, detected by HPLC-DAD, varied by sites with a range from undetectable concentrations to 0.46 µg L−1 with only one recorded variant, namely MC-LR. It was found only in the reservoirs Mandra and Durankulak, while toxigenic MC-strains were obtained by PCR from five more waterbodies. Both LM and PCR demonstrated that the MC-producers were Microcystis aeruginosa and M. wesenbergii, despite their occurrence in low amounts (<0.5–5% of the total biomass) when filamentous cyanoprokaryotes dominated.
不断变化的世界中的有害藻华：对有害藻华、其影响以及气候和环境变化动态时代的研究和管理的看法。
DOI：
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发表时间：
2014
期刊：
Harmful algae 2012 : proceedings of the 15th International Conference on Harmful Algae : October 29 - November 2, 2012, CECO, Changwon, Gyeongnam, Korea. International Conference on Harmful Algae (15th : 2012 : Changwon, Gyeongnam, Kore...
影响因子：
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作者：
Anderson,Donald
通讯作者：
Anderson,Donald