Simultaneous Determination of Ten Active Components in Chinese Medicine “Huang-Lian-Shang-Qing” Tablets by High-Performance Liquid Chromatography Coupled with Photodiode Array Detection
Simultaneous Determination of Ten Active Components in Chinese Medicine “Huang-Lian-Shang-Qing” Tablets by High-Performance Liquid Chromatography Coupled with Photodiode Array Detection
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DOI：
10.1080/00032710903406847
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发表时间：
2010-02
影响因子：
2
通讯作者：
Xu Han;L. Yin;Lina Xu;Xiuying Wang;Jinyong Peng
中科院分区：
文献类型：
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作者：
Xu Han;L. Yin;Lina Xu;Xiuying Wang;Jinyong Peng
A simple and sensitive high-performance liquid chromatography coupled with a photodiode array detection (HPLC-PAD) method was investigated for the simultaneous determination of ten components (rutin, berberine, palmatine, baicalin, wogonoside, baicalein, wogonin, emodin, chrysophanol, and physcion) in “Huang-Lian-Shang-Qing” (HLSQ) tablets. The method was optimized and the mobile phase composed of methanol (A)-3% phosphoric acid (B) was used to elute the targets in a gradient elution mode. All the calibration curves, precisions, and recoveries were good. Then, this method was successfully used to determine the ten compounds in 33 batches of HLSQ tablets for quality control of this medicinal product.
