Specific types of human papillomavirus found in benign proliferations and carcinomas of the skin in immunosuppressed patients.
Specific types of human papillomavirus found in benign proliferations and carcinomas of the skin in immunosuppressed patients.
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在免疫抑制患者的良性增殖和皮肤癌中发现了特定类型的人乳头瘤病毒。
作者：
V. Shamanin;M. Glover;Christine Rausch;C. Proby;I. Leigh;H. Hausen;E. Villiers
共118例皮肤病变活检的46例肾移植患者进行了分析，人乳头瘤病毒（HPV）DNA聚合酶链反应与简并引物，也部分通过随后的测序扩增片段。62%的良性增生（50例中的31例）含有已知HPV类型的DNA以及与许多疣状表皮发育不良相关的HPV类型相关的HPV序列。在25例鳞状细胞癌和基底细胞癌活检标本中，14例（56%）发现了HPV DNA序列。1例鳞状细胞癌含有HPV 41 DNA。在15%（3/20）的鳞状细胞癌、9.4%（3/32）的异型增生疣和8.5%（4/47）的普通疣中发现了一种与HPV 29（82.7%）具有同源性的新的640碱基对片段。剩余的阳性癌活检标本含有HPV相关DNA，其拷贝数如此之低，需要进行额外的分析。免疫抑制患者（疣状表皮发育不良患者除外）皮肤癌中新HPV类型的鉴定进一步扩大了HPV相关人类恶性肿瘤的范围，并允许研究皮肤癌发病机制的新方法。
A total of 118 biopsies from skin lesions of 46 renal allograft patients was analyzed for human papillomavirus (HPV) DNA by polymerase chain reaction with degenerate primers and also partially by subsequent sequencing of the amplified fragment. Sixty-two % of the benign proliferations (31 of 50) contained DNA of known HPV types as well as HPV sequences related to a number of epidermodysplasia verruciformis-associated HPV types. HPV DNA sequences were found in 14 (56%) of 25 biopsies from squamous cell and basal cell carcinomas. One squamous cell carcinoma contained HPV 41 DNA. A novel 640-base pair fragment sharing homology with HPV 29 (82.7%) was found in 15% (3 of 20) of squamous cell carcinomas, in 9.4% (3 of 32) of dysplastic warts and in 8.5% (4 of 47) common warts. The remaining positive carcinoma biopsies contained HPV-related DNA in such a low copy number that additional analysis is required. The identification of new HPV types in skin cancers of immunosuppressed patients (other than epidermodysplasia verruciformis patients) further expands the spectrum of HPV-linked human malignancies and permits new approaches to study the pathogenesis of skin cancers.