Killing of Gram-positive cocci by fatty acids and monoglycerides
Killing of Gram-positive cocci by fatty acids and monoglycerides
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DOI：
10.1034/j.1600-0463.2001.d01-131.x
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发表时间：
2001-10-01
期刊：
影响因子：
2.8
通讯作者：
Thormar, H
中科院分区：
文献类型：
--
作者：
Bergsson, G;Arnfinnsson, J;Thormar, H
研究了三种革兰氏阳性球菌对中链饱和脂肪酸和长链不饱和脂肪酸及其单甘酯的敏感性。将细菌与等体积的脂质溶液一起孵育10 min。对于A组（GAS）和B组（GBS）的链球菌，月桂酸、棕榈油酸和单癸酸甘油酯在5 mM浓度下可使CFU数量减少6.0 log（10）或更多。当在较低浓度和较长孵育时间后进一步比较时，证明单癸酸甘油酯最具活性。癸酸在10 mM时对金黄色葡萄球菌显示出最高活性。然而，在较低浓度下，单癸酸甘油酯是唯一对金黄色葡萄球菌显示出显著活性的脂质。金黄色。单癸酸甘油酯对GBS的作用模式进行了研究，通过一种新的双色荧光测定细菌活力和电子显微镜。结果表明，细菌通过脂质分解细胞膜而被杀死，留下完整的细菌细胞壁。单癸酸甘油酯的高致死作用表明，这种脂质可能用作杀微生物剂，用于预防和治疗由这些细菌引起的感染。
The susceptibilities of three Gram-positive cocci to medium-chain saturated and long-chain unsaturated fatty acids and their one-monoglycerides were studied. The bacteria were incubated with equal volumes of lipid solutions for 10 min. Lauric acid, palmitoleic acid and monocaprin reduced the number of CFU by 6.0 log(10) or greater at 5 mM concentration for streptococci of group A (GAS) and group B (GBS). When further compared at lower concentrations and after longer incubation time monocaprin proved to be the most active. Capric acid showed the highest activity against Staphylococcus aureus at 10 mM. However, at lower concentrations monocaprin was the only lipid that showed significant activity against S. aureus. The mode of action of monocaprin against GBS was studied by a novel two-color fluorescent assay of bacterial viability and by electron microscopy. The results, indicate that the bacteria are killed by disintegration of the cell membrane by the lipid, leaving the bacterial cell wall intact. The highly lethal effect of monocaprin indicates that this lipid might be useful as a microbicidal agent for prevention and treatment of infections caused by these bacteria.