ACTIVATED H-RAS TRANSFORMS RAT INTESTINAL EPITHELIAL-CELLS WITH EXPRESSION OF ALPHA-TGF
ACTIVATED H-RAS TRANSFORMS RAT INTESTINAL EPITHELIAL-CELLS WITH EXPRESSION OF ALPHA-TGF
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DOI：
10.1016/0014-4827(87)90308-9
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发表时间：
1987-06-01
影响因子：
3.7
通讯作者：
QUARONI, A
中科院分区：
文献类型：
--
作者：
BUICK, RN;FILMUS, J;QUARONI, A
我们描述了通过用从 EJ 膀胱癌克隆的激活的人 H-ras 基因转染培养的大鼠肠上皮细胞 (IEC-18) 的恶性转化。转化细胞表现出明显的形态变化，表达高水平的转染H-ras基因，能够在琼脂中生长，并表达与亲本IEC-18细胞相同的抗原标记。当注射到同基因大鼠体内时，这些细胞形成快速生长的肿瘤，表达与注射细胞相同的肠道特异性抗原标记。与转化体中H-ras mRNA的高表达平行，我们记录了大鼠α-TGF mRNA的过度表达。
We describe malignant transformation of cultured rat intestinal epithelial cells (IEC-18) by transfection with activated human H-ras gene cloned from the EJ bladder carcinoma. Transformed cells showed a marked morphological change, expressed high levels of the transfected H-ras gene, were able to grow in agar and expressed antigenic markers identical with parental IEC-18 cells. When injected into syngeneic rats these cells formed rapidly growing tumors expressing the same intestinal-specific antigenic markers as the injected cells. Parallel to the high expression of H-ras mRNA in the transformants we document overexpression of rat .alpha.-TGF mRNA.