Identification of paternal mitochondrial DNA sequences in the nucleus–cytoplasm hybrids of tetraploid and hexaploid wheat with D and D2 plasmons from Aegilops species
Identification of paternal mitochondrial DNA sequences in the nucleus–cytoplasm hybrids of tetraploid and hexaploid wheat with D and D2 plasmons from Aegilops species
复制标题
具有来自山羊草物种的 D 和 D2 等离子体的四倍体和六倍体小麦的核-细胞质杂种中父本线粒体 DNA 序列的鉴定
DOI：
10.1007/s002940000153
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发表时间：
2000
期刊：
影响因子：
2.5
通讯作者：
C. Nakamura
中科院分区：
文献类型：
--
作者：
Naoko Tsukamoto;N. Asakura;Nobuaki Hattori;S. Takumi;N. Mori;C. Nakamura
摘要对12个四倍体和六倍体小麦核质杂种的线粒体DNA结构进行了研究。对15个线粒体DNA区域的RFLP分析表明，在所有NC杂种中，包含11个结构基因的6个区域中存在父系序列。PCR-RFLP分析表明，一个区域（nad 3-orf 156区域）由母本、父本和新的父本样序列的混合物组成。通过该区域的随机PCR克隆和测序证实了出乎意料的高水平父本序列的存在。随机克隆的PCR-RFLP分析进一步表明，母本和父本序列的相对化学计量根据来自母本的等离子体和父本的核背景而变化。我们的研究结果表明，在这些NC杂种中，父本mtDNA序列的差异扩增是在NC相互作用的控制下。
Abstract Mitochondrial (mt) DNA structures were studied in 12 nucleus–cytoplasm (NC) hybrids of tetraploid and hexaploid wheat in which nuclear genomes of the recurrent paternal wheat parents were combined with D and D2 plasmons from Aegilops species. RFLP analysis of 15 mtDNA regions indicated the presence of the paternal sequences in six regions encompassing 11 structural genes in all the NC hybrids. PCR-RFLP analysis showed that one region (a nad3-orf156 region) consisted of a mixture of the maternal, paternal and novel paternal-like sequences. The presence of unexpectedly high levels of the paternal sequences was confirmed by random PCR cloning and sequencing of this region. PCR-RFLP analysis of the random clones further showed that the relative stoichiometry of the maternal and paternal sequences varied depending on the plasmons from the maternal parents and the nuclear backgrounds of the paternal parents. Our results suggest that the differential amplification of the paternal mtDNA sequences is under the control of NC interaction in these NC hybrids.