Characterization of Depth-Related Changes in Bacterial Communities Involved in Mineral Weathering Along a Mineral-Rich Soil Profile
Characterization of Depth-Related Changes in Bacterial Communities Involved in Mineral Weathering Along a Mineral-Rich Soil Profile
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DOI：
10.1080/01490451.2013.848248
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发表时间：
2014-03
影响因子：
2.3
通讯作者：
Qi Wang;Cheng Cheng-Cheng;Linyan He;Zhi Huang;X. Sheng
中科院分区：
文献类型：
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作者：
Qi Wang;Cheng Cheng-Cheng;Linyan He;Zhi Huang;X. Sheng
在这项研究中，我们使用变性梯度凝胶电泳(DGGE)和依赖于培养的方法来表征富含矿物质的土壤剖面中的细菌种群和矿物溶解细菌。DGGE和测序揭示了土壤剖面的13个已知细菌科和7个未知种群。有71株菌株对长石有增溶作用。不同层位菌株的风化效果和风化格局不同。71株溶矿菌株隶属于14属32种细菌，其中芽孢杆菌、伯克霍尔德氏菌和节杆菌为优势种。在表层和次表层之间观察到了不同的矿物溶解种群。值得注意的是，最深的土层显示了溶解矿物的细菌的最大多样性。此外，在较深层观察到的高效溶矿细菌的比例明显高于较高层。结果表明，土壤剖面中存在着不同的溶矿种群，溶矿细菌的溶矿潜力、溶矿格局和群落结构随深度的变化而变化。
In this study, we used denaturing gradient gel electrophoresis (DGGE) and culture-dependent methodology to characterize bacterial populations and mineral-dissolving bacteria in a mineral-rich soil profile. DGGE and sequencing revealed 13 known bacterial families and 7 unknown populations for the soil profile. Seventy-one isolates could solubilize feldspar. Weathering effectiveness and pattern of the isolates differed among the horizons. The 71 mineral-dissolving isolates were affiliated with 32 bacterial species within 14 genera, among which Bacillus, Burkholderia, and Arthrobacter were dominant. Distinct mineral-dissolving populations were observed between the surface and subsurface horizons. Notably, the deepest horizon showed maximum diversity of the mineral-dissolving bacteria. Furthermore, a significantly higher proportion of the high efficiency mineral-dissolving bacteria was observed in the deeper horizons than in the upper horizons. The results suggested that the soil profile harboured diverse mineral-dissolving populations and the dissolving potential and pattern and the community of the mineral-dissolving bacteria changed with depth.