Total retinal blood flow measurement with ultrahigh speed swept source/Fourier domain OCT.
Total retinal blood flow measurement with ultrahigh speed swept source/Fourier domain OCT.
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DOI：
10.1364/boe.2.001539
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发表时间：
2011-06-01
影响因子：
3.4
通讯作者：
Fujimoto JG
中科院分区：
文献类型：
--
作者：
Baumann B;Potsaid B;Kraus MF;Liu JJ;Huang D;Hornegger J;Cable AE;Duker JS;Fujimoto JG
多普勒OCT可提供生物组织中血流的深度分辨信息。在这篇文章中，我们展示了高速扫描源/傅立叶域OCT的可视化和定量评估视网膜血流。使用扫描激光技术，该系统在1050 nm波长范围内以200 kHz的高轴向扫描速率运行。快速的成像速度不仅能够实现具有高轴向扫描密度的体积成像，而且还能够测量中央视网膜血管中的高流速。通过1 µm波长成像实现视神经和筛板的深度穿透。通过分析从3D多普勒数据集提取的正面图像，使用不需要测量多普勒角度的简单且鲁棒的协议测量单血管中的绝对流量以及总视网膜血流。健康眼睛的测量结果表明，高速扫频源/傅立叶域OCT可能是一种很有前途的技术，用于视网膜血管的体积成像和定量视网膜血流在广泛的视网膜疾病。
Doppler OCT provides depth-resolved information on flow in biological tissues. In this article, we demonstrate ultrahigh speed swept source/Fourier domain OCT for visualization and quantitative assessment of retinal blood flow. Using swept laser technology, the system operated in the 1050-nm wavelength range at a high axial scan rate of 200 kHz. The rapid imaging speed not only enables volumetric imaging with high axial scan densities, but also enables measurement of high flow velocities in the central retinal vessels. Deep penetration in the optic nerve and lamina cribrosa was achieved by imaging at 1-µm wavelengths. By analyzing en-face images extracted from 3D Doppler data sets, absolute flow in single vessels as well as total retinal blood flow was measured using a simple and robust protocol that does not require measurement of Doppler angles. The results from measurements in healthy eyes suggest that ultrahigh speed swept source/Fourier domain OCT could be a promising technique for volumetric imaging of retinal vasculature and quantitation of retinal blood flow in a wide range of retinal diseases.