Validation of the cytokinesis-block micronucleus (CBMN) assay for use as a triage biological dosimetry tool
Validation of the cytokinesis-block micronucleus (CBMN) assay for use as a triage biological dosimetry tool
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DOI：
10.1093/rpd/ncp119
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发表时间：
2009-08-01
影响因子：
1
通讯作者：
Wilkins, Ruth C.
中科院分区：
文献类型：
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作者：
McNamee, James P.;Flegal, Farrah N.;Wilkins, Ruth C.
传统上，双中心染色体测定（DCA）已被用于推算未知辐射照射的生物剂量。虽然敏感，但这种分析需要训练有素的评估人员，而且非常耗时。细胞分裂阻滞微核（CBMN）测定被建议作为DCA的替代方法，因为它评估样品的速度要快得多，并且需要较少的技术专业知识。为了验证该方法在分诊生物剂量学中的应用，对6名供体在8次γ辐射剂量（0-4.0 Gy）下的剂量-反应曲线进行了生成。每个样本由12个人在三个不同的实验室中进行评估，在计数50-500个双核细胞后确定微核的发生率。本研究表明，CBMN法仅检测200个双核细胞，即可检测辐射剂量>= 1 Gy。因此，在需要进行大量生物剂量估计的大规模放射紧急情况下，CBMN测定可作为初始筛选工具，提供一种敏感而可靠的技术。
Traditionally, the dicentric chromosome assay (DCA) has been used to derive biological dose estimates for unknown radiological exposures. While sensitive, this assay requires highly trained evaluators and is extremely time consuming. The cytokinesis-block micronucleus (CBMN) assay has been suggested as an alternative to the DCA, as it is much faster to evaluate samples and requires less technical expertise. In order to validate this assay for triage biodosimetry, dose-response curves were generated for six donors at eight doses of gamma-radiation (0-4.0 Gy). Each sample was evaluated by 12 individuals, among three different laboratories and the incidence of micronuclei was determined after counting 50-500 binucleated cells. This study demonstrated that the CBMN assay was capable of detecting radiation doses >= 1 Gy after scoring only 200 binucleated cells. As such, the CBMN assay may provide a sensitive and reliable technique for deployment as an initial screening tool in a large-scale radiological emergency where large numbers of biological dose estimates are required.