Correlation of serum HIV antigen and antibody with clinical status in HIV‐I infected patients
Correlation of serum HIV antigen and antibody with clinical status in HIV‐I infected patients
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HIV-I感染者血清HIV抗原和抗体与临床状态的相关性
DOI：
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发表时间：
1987
影响因子：
12.7
通讯作者：
A. Landay
中科院分区：
文献类型：
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作者：
D. Paul;L. Falk;H. Kessler;R. M. Chase;B. Blaauw;D. Chudwin;A. Landay
已开发出一种酶免疫测定法（EIA），用于检测获得性免疫缺陷综合征（AIDS）、AIDS相关复合物（ARC）患者和HIV感染高危患者血清中的人类免疫缺陷病毒（HIV）抗原（Ag）。该测试具有约50 pg/ml的HIV蛋白的灵敏度。使用各种病毒感染细胞系、正常人血清/血浆和来自未知HIV感染风险的患者的血清测定了该试验的特异性。未观察到假阳性HIV‐Ag结果。69%的艾滋病患者的血清中HIV-抗原呈阳性，46%的ARC患者和19%的无症状HIV抗体阳性者也是如此。HIV感染阶段（即AIDS vs ARC vs无症状）与血清中HIV-Ag检测（p < 0.0001）和HIV核心抗原抗体检测缺失（p < 0.0001）之间存在显著相关性。在两名无症状抗体阴性个体的血清中也发现了HIV-Ag，这两名个体具有艾滋病的高风险，后来产生了HIV抗体。通过抑制程序确认血清中存在HIV‐Ag。因此。HIV-Ag可以在抗体产生之前在感染个体的血清中检测到，并与HIV感染的临床阶段相关。
An enzyme immunoassay (EIA) has been developed which detects antigen(s) (Ag) of the human immunodeficiency virus (HIV) in the serum of patients with the acquired immunodeficiency syndrome (AIDS), AIDS‐related complex (ARC), and patients at high risk for HIV infection. The test has a sensitivity of approximately 50 pg/ml of HIV protein. The specificity of the assay was determined with various virus infected cell lines, normal human sera/plasma, and serum from patients not known to be at risk for HIV infection. No false‐positive HIV‐Ag results were seen. Sera from 69% of patients with AIDS were positive for HIV‐Ag as were 46% of patients with ARC and 19% of asymptomatic, HIV‐antibody‐positive individuals. There were significant associations between the stage of HIV infection–ie, AIDS vs ARC vs asymptomatic‐and the detection of HIV‐Ag in serum (p < 0.0001) and the lack of detection of antibody to HIV core Ag (p < 0.0001). HIV‐Ag was also found in the serum of two asymptomatic antibody‐negative individuals who were at high risk for AIDS and who later developed HIV antibody. The presence of HIV‐Ag in sera was confirmed by an inhibition procedure. Thus. HIV‐Ag can be detected in the serum of infected individuals prior to antibody production and correlates with the clinical stage of HIV infection.