Organic Nanoprobe Cocktails for Multilocal and Multicolor Fluorescence Imaging of Reactive Oxygen Species
Organic Nanoprobe Cocktails for Multilocal and Multicolor Fluorescence Imaging of Reactive Oxygen Species
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DOI：
10.1002/adfm.201700493
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发表时间：
2017-06
影响因子：
19
通讯作者：
Chao Yin;Houjuan Zhu;Chen Xie;Lei Zhang;Peng Chen;Quli Fan;Wei Huang;Kanyi Pu
中科院分区：
文献类型：
--
作者：
Chao Yin;Houjuan Zhu;Chen Xie;Lei Zhang;Peng Chen;Quli Fan;Wei Huang;Kanyi Pu
Hypochlorite (ClO−) as a highly reactive oxygen species not only acts as a powerful “guarder” in innate host defense but also regulates inflammation‐related pathological conditions. Despite the availability of fluorescence probes for detection of ClO− in cells, most of them can only detect ClO− in single cellular organelle, limiting the capability to fully elucidate the synergistic effect of different organelles on the generation of ClO−. This study proposes a nanoprobe cocktail approach for multicolor and multiorganelle imaging of ClO− in cells. Two semiconducting oligomers with different π‐conjugation length are synthesized, both of which contain phenothiazine to specifically react with ClO− but show different fluorescent color responses. These sensing components are self‐assembled into the nanoprobes with the ability to target cellular lysosome and mitochondria, respectively. The mixture of these nanoprobes forms a nano‐cocktail that allows for simultaneous imaging of elevated level of ClO− in lysosome and mitochondria according to fluorescence color variations under selective excitation of each nanoprobe. Thus, this study provides a general concept to design probe cocktails for multilocal and multicolor imaging.
